摘要
【目的】建立高辣度辣椒的高效离体培养再生体系,为其工厂化育苗生产奠定基础。【方法】以云南宏绿辣素有限公司选育的高辣度辣椒品种宏绿1号种子为试材获得无菌外植体,采用单因素试验和正交试验设计对基本培养基、芽诱导分化、增殖、生根培养及移栽等影响因子进行研究。【结果】不定芽诱导的适宜基本培养基为MS+30 g/L蔗糖+7 g/L琼脂;筛选出MS+2.5 mg/L 6-BA+0.05 mg/L NAA+5 mg/L AgNO3为不定芽分化的最佳培养基,分化率达93.3%;在MS+5 mg/L 6-BA+0.1 mg/L NAA培养基上进行不定芽茎伸长诱导,伸长率可达100%;最适生根培养基为B5+30 g/L蔗糖+7 g/L琼脂,生根率达100%,炼苗移栽后成活率可达98.0%。【结论】建立了该辣椒品种高效的离体快繁培养技术体系。
[Purpose]The establishment of efficient regeneration system by in vitro culture of hot pepper can lay the foundation for its industrial seedling production.[Method]Sterile explants were obtained from the seeds of ‘Honglyu No.1’ pepper from Yunnan Honglyu Capsaicin Co., Ltd. and the single-factor design and orthogonal design were employed to examine the effect of initiation medium,adventitious bud differentiation, proliferation, rooting culture and transplantation on this system.[Result]The optimal initiation culture medium for adventitious bud induction was MS + 30 g/L sucrose + 7 g/L agar. MS + 2.5 mg/L 6-BA + 0.05 mg/L NAA + 5 mg/L AgNO3 was optimal to adventitious bud differentiation and its differentiation rate was 93.3%. The bud elongation ratio was100% when it was induced on MS + 5 mg/L 6-BA + 0.1 mg/L NAA. Rooting rate 100% of adventitious buds was obtained on B5 + 30 g/L sucrose + 7 g/L agar. After seedling adaptation, the survival rate of regenerated plants was 98% when seedlings were transplanted into substrate.[Conclusion]The research established the rapid in vitro propagation system of ‘Honglyu No.1’.
作者
钟源源
李思琦
何小帆
李彪
肖丰坤
何承忠
ZHONG Yuanyuan;LI Siqi;HE Xiaofan;LI Biao;XIAO Fengkun;HE Chengzhong(College of Forestry,Southwest Forestry University,Kunming 650224,China;Yunnan Honglyu Capsaicin Co.,Ltd.,Kunming 650500,China;College of Life Science,Southwest Forestry University,Kunming 650224,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2019年第6期1041-1047,共7页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省教育厅重大专项（ZD2013014）
关键词
高辣度辣椒
不定芽分化
植株再生
hot pepper
adventitious bud differentiation
plant regeneration
作者简介
钟源源(1994—),女,云南昭通人,在读硕士研究生,主要从事植物繁殖技术研究。E-mail:zhongyuanyuan221@sina.com;通信作者:何承忠(1970—),男,甘肃民勤人,博士,教授,博士研究生导师,主要从事林木遗传育种与分子生物学研究。E-mail:hcz70@163.com。
