摘要
为给山苍子组培快繁体系的建立提供参考,以山苍子春梢和秋梢的带芽茎段作为外植体,进行不同消毒时间和消毒剂组合处理,并对其死亡率进行分析。结果表明,山苍子春梢(3月中旬—4月中旬)带芽茎段,经流水冲洗60 min后,用75%的乙醇消毒25 s,随后用0.1%的升汞消毒4 min,接种30 d后其生长状态最好,存活率为80%。山苍子秋梢(9月中旬—10月中旬)带芽茎段,需较长时间的消毒处理,带芽茎段经流水冲洗3~6 h后,用75%的乙醇消毒40 s,随后用0.1%的升汞消毒12 min,接种30 d后其存活率最高为38.37%。
In order to provide some references for establishment of rapid propagation system of Litsea cubeba,stem segments with buds of spring and autumn shoots in L.cubeba were used as explants,and mortality was researched through adopting combination treatments of different disinfection time and disinfectants.The results showed that inoculated stem segments with buds of spring shoots in L.cubeba(from mid-March to mid-April)had the best growth status after 30 d,which were rinsed with running water for 60 min,and then were disinfected with 75%ethanol for 25 s and 0.1%mercuric chloride for 4 min,and survival rate was 80%.Stem segments with buds of autumn shoots in L.cubeba(from mid-September to mid-October)need a longer period of disinfection.Inoculated stem segments with buds of autumn shoots had the highest survival rate(38.37%)after 30 d,which were rinsed with running water for 3 to 6 h and then were disinfected with 75%ethanol for 40 s and 0.1%mercury for 12 min.
作者
张付豪
李佳洛
王阳
许婷
陈昊
ZHANG Fuhao;LI Jialuo;WANG Yang;XU Ting;CHEN Hao(Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education,Central South University of Forestry&Technology,Changsha 410004,Hunan,China)
出处
《经济林研究》
北大核心
2019年第4期204-209,共6页
Non-wood Forest Research
基金
湖南省重点研发计划项目(2018NK2044)
关键词
山苍子
外植体
带芽茎段
消毒方法
Litsea cubeba
explants
stem segment with buds
disinfection methods
作者简介
张付豪,硕士研究生;通信作者:陈昊,副教授,博士。E-mail:chenhao198223@sina.com。
