摘要
为了探明黄淮稻区4个抗稻瘟病基因Pi-ta、Pi-b、Pi54和Pikm在品种资源中的分布及其不同基因型组合的抗病效性。本研究利用上述4个抗病基因的功能标记,对88个黄淮稻区育成品种进行抗稻瘟病基因型检测,结果表明:检出最多的抗稻瘟病基因组合是Pib+Pi54,占总检测材料的60.2%。经过2016-2017年连续两年的人工接种鉴定和田间自然鉴定,稻瘟病感病材料比例分别达到87.5%、77.3%,感病材料的比例增高说明抗性基因的抗性作用在逐渐降低。在检测品种中,抗性较好的4个基因型或基因型组合分别是Pi-ta、Pi-ta+Pi-b、Pi-ta+Pi-b+Pi54和Pi-ta+Pi-b+Pi54+Pikm,但检出率很低。本研究结果说明,黄淮稻区品种中,携带Pi-ta、Pi-ta+Pi-b、Pi-ta+Pi-b+Pi54和Pi-ta+Pi-b+Pi54+Pikm基因型的抗病性较好,应得到加强利用。本研究结果对育成品种抗稻瘟病基因或基因组合跟踪检测和抗病性评价是抗稻瘟病育种的有效途径。
To clarify the distribution status of Pi-ta,Pi-b,Pi54,Pikm genes and their effects on the resistance of rice blast in Huang-Huai-Hai Region,88 rice varieties collected from this region were detected using the functional markers that specifically target to the four genes.The genetic frequency of Pib+Pi54 was 60.2% within this collection.In parallel,this collection was subjected to test for resistance by inoculation identification and field identification in 2016 and 2017.87.5% and 77.3% of varieties were susceptible in two years,respectively.This result suggested that the effects of resistant genes were declined.Four genes or gene combinations(Pi-ta,Pi-ta+Pi-b,Pi-ta+Pi-b+Pi54 and Pi-ta+Pi-b+Pi54+Pikm)are applicable in the rice-breeding,due to the resistance to rice blast but with low distribution in the tested varieties.Taken together,the results raised a positive strategy for breeding rice cultivars with rice blast-resistance.
作者
王宝祥
刘艳
邢运高
迟铭
徐波
杨波
李健
孙志广
刘金波
陈庭木
卢百关
方兆伟
徐大勇
WANG Bao-xiang;LIU Yan;XING Yun-gao;CHI Ming;XU Bo;YANG Bo;LI Jian;SUN Zhi-guang;LIU Jin-bo;CHEN Ting-mu;LU Bai-guan;FANG Zhao-wei;XU Da-yong(Lianyungang Academy of Agricultural Sciences/Jiangsu Collaborative Innovation Center for Modern Corp Production,Lianyungang 222006)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第6期1465-1471,共7页
Journal of Plant Genetic Resources
基金
国家七大育种专项(2017YFD0100400)
江苏省自然科学基金(BK20161299)
江苏省科技支撑项目(BE2017323)
江苏省农业重大品种创制(PZCZ201704)
连云港市财政专项(QNJJ201712)~~
关键词
水稻
稻瘟病
基因型
育种
rice
rice blast
genotype
rice-breeding
作者简介
第一作者:王宝祥,研究方向为水稻遗传育种,E-mail:wbxrice@163.com;通信作者:徐大勇,研究方向为农作物新品种选育和推广,E-mail:xudayong3030@sina.com。
