摘要
利用具有聚集诱导发光特性的荧光染料4,4'-[(1E,1'E)蒽-9,10-二基双(乙烯-2,1-二基)]双(N,N-二甲基苯胺)(NDSA),通过两亲性聚合物二硬脂酰基磷脂酰乙醇胺-聚乙二醇-N-羟基琥珀酰亚胺(DSPE-PEGNHS)包覆的方法制备了明亮的橙色荧光纳米粒子,其最大发射波长为559 nm,在水溶液中具有2. 89%的荧光量子产率.该纳米粒子具有优异的发光特性和良好的生物相容性.在该纳米粒子表面修饰肝癌细胞靶向的人类婆罗双树样基因-4(SALL4)抗体后,荧光纳米粒子NDSA@SALL4可以特异性地靶向肝癌细胞,还可以在细胞核富集,呈现出明亮的橙色荧光,为早期检测肝癌细胞提供了可能.
A bright orange fluorescent nanoparticles NDSA@SALL4[NDSA=4,4′ (1E,1′E) 2,2 (anthra cene 9,10 diyl)bis(ethane 2,1 diyl)bis(N,N dimethylaniline),SALL4=Sal like protein 4]encapsulated by amphiphilic polymer 1,2 distearoyl sn glycero 3 phosphoethanolamine N [succinimidyl(polyethylene glycol)](DSPE PEG NHS)was prepared,which further modified SALL4 antibody.The nanoparticles NDSA@SALL4 shows good biocompatibility and high photoluminescence,which peaked at 559 nm and exhibited a fluorescence quantum yield of 2.89% in aqueous medium.NDSA@SALL4 can not only specifically target liver cancer cells,but also accurately image the nucleus of cell with bright orange fluorescence,demonstrating that they have a good candidate for early diagnose and bioimaging applications.
作者
张雨
荆江博
邵玥明
殷鑫
徐斌
温晓玉
ZHANG Yu;JING Jiangbo;SHAO Yueming;YIN Xin;XU Bin;WEN Xiaoyu(The First Hospital of Jilin University,Changchun 130021,China;State Key Laboratory of Supramolecular Structure and Materials,Jilin University,Changchun 130012,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2019年第11期2382-2387,共6页
Chemical Journal of Chinese Universities
基金
吉林省自然科学基金(批准号:20190201065JC)资助~~
作者简介
联系人:温晓玉,女,博士,副教授,主要从事原发性肝癌研究.E mail:15804301609@163.com。
