摘要
目的:利用模式动物斑马鱼研究自噬对其尾鳍再生的影响。方法:选取成年斑马鱼剪去尾鳍,随后腹腔注射自噬抑制剂3-甲基腺嘌呤(3-Methyladenine,3-MA)或诱导剂STF-62247,连续注射2天,剪取再生尾鳍,通过qPCR分析自噬相关基因ATG和Beclin-1的表达。斑马鱼剪去尾鳍,连续7天腹腔注射3-MA或STF-62247,每天记录斑马鱼尾鳍再生长度。结果:尾鳍再生过程中,3-MA下调ATG和Beclin-1表达,STF-62247提高ATG表达,抑制自噬或诱导自噬对斑马鱼尾鳍再生速度无显著影响。结论:腹腔注射自噬抑制剂3-MA或自噬诱导剂STF-62247对斑马鱼尾鳍再生速度无显著影响。
Objective:To explore the effects of autophagy on zebrafish caudal fin regeneration.Methods:Adult zebrafish caudal fins amputated with were selected.Then,the adult zebrafish was injected with the autophagy inhibitor 3-Methyladenine(3-MA)or the activator STF-62247 once a day for two consecutive days.The regenerated caudal fin was collected to analyze the expressions of autophagy related genes ATG and Beclin-1 by using qPCR.Furthermore,adult zebrafish were amputated and randomly divided into the control group,the 3-MA injected group and the STF-62247 injected group.The autophagy inhibitor 3-MA or the activator STF-62247 was injected for 7 consecutive days.The length of regenerated zebrafish caudal fin was recorded every day.The effects of autophagy inhibition or autophagy activation on the regeneration rate of zebrafish caudal fin were analyzed.Results:During the caudal fin regeneration,the expression of ATG and Beclin-1 was down-regulated by 3-MA,while STF-62247 enhanced ATG and Beclin-1 expression levels.Compared with the control group,both inhibition of autophagy and activation of autophagy showed no significant effects on the rate of zebrafish caudal fin regeneration.Conclusion:Through intraperitoneal injection,neither autophagy inhibitor 3-MA nor autophagy activator STF-62247 exhibits any significant effects on the regeneration rate of zebrafish caudal fin.
作者
陈家宁
王晓宁
郏科人
申屠瑜婷
段旭初
石运伟
刘东
胡松群
CHEN Jianing;WANG Xiaoning;JIA Keren;SHENTU Yuting;DUAN Xuchu;SHI Yunwei;LIU Dong;HU Songqun(School of Medicine,Nantong University,Jiangsu 226001;School of Life Sciences,Nantong University,Jiangsu 226001;Key Laboratory of Neuroregeneration,Nantong University,Jiangsu 226001;Affiliated Hospital of Nantong University)
出处
《交通医学》
2019年第5期429-432,422,共4页
Medical Journal of Communications
基金
国家自然科学基金(201870359)
南通市科技计划项目(MS12017012-4,MS12017015-6)
江苏省大学生创新创业训练计划项目(201910304060Z)
关键词
自噬
斑马鱼
尾鳍再生
autophagy
zebrafish
caudal fin regeneration
作者简介
陈家宁,男,汉族,江苏南通人,生于1996年10月,本科生。研究方向:尾鳍再生;通信作者:胡松群,E-mail:hsq@ntu.edu.cn。
