摘要
本研究以九种青岛市售贝类样品为研究对象,并且通过高效液相色谱-碰撞/反应池-电感耦合等离子体质谱(HPLC-CRC-ICP-MS)测定总砷及砷形态化合物的含量,并对其食用安全性做出客观评价。样品采用超声提取(0.15 mol/L HNO3),优化后的色谱条件(50 mmol/L(NH4)2CO3,pH=9.5)进行色谱分离,并采用碰撞/反应池中O2反应模式进行检测。6种砷形态化合物的线性回归方程相关系数均在0.999以上,检出限为0.009~0.028μg/L,相对标准偏差在3.6%~8.3%之间,加标回收率在91.2%~106.2%。实验表明,青岛市售贝类样品中的砷元素主要以无毒性的AsB的形式存在,无机砷含量符合国标限量要求。
This study aimed at developing an accurate method for determining the levels of total arsenic and arsenic species in edible species of shellfish using high performance liquid chromatography coupled with collision/reaction cell inductively coupled plasma mass spectrometry to assess the healthy risk objectively.The shellfish samples were extracted using ultrasonic bath extraction(0.15 mol/L HNO3),separated under the optimized chromatographic conditions(50 mmol/L(NH4)2CO3,pH=9.5)and determined with O2 mass-shift mode.Good linearities of six arsenic species were observed with correlation coefficients above 0.999.Limits of detection were between 0.009~0.028μg/L.Relative standard deviation were within 3.6%~8.3%and recoveries were between 91.2%~106.2%.The results showed that AsB was the predominant as species in the shellfish samples of Qingdao City and inorganic arsenic contents meets the international limit.
作者
王志鹏
薛长湖
徐杰
李兆杰
WANG Zhi-peng;XUE Chang-hu;XU Jie;LI Zhao-jie(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Qingdao National Laboratory for Marine Science and Technology,Qingdao 266003,China)
出处
《食品工业科技》
CAS
北大核心
2019年第17期244-250,共7页
Science and Technology of Food Industry
基金
国家贝类产业体系(CARS-47)
作者简介
王志鹏(1995-),男,硕士研究生,研究方向:水产品中重金属形态分析,E-mail:wzp1776716658@163.com;通讯作者:薛长湖(1964-),男,博士,教授,主要从事水产品加工与贮藏工程学科的水产化学与水产品加工技术方面的研究,E-mail:xuech@ouc.edu.cn。
