摘要
目的探讨白细胞介素1受体拮抗剂(IL-1RA)对慢性阻塞性肺疾病(COPD)模型大鼠气道炎症和气道重塑的保护作用。方法采用烟熏加气管内注射脂多糖(LPS)对30只Wistar大鼠造模,并采用随机数字表法将其分为对照组、甲泼尼龙治疗组、IL-1RA治疗组,每组10只。于第20天开始对各组大鼠连续干预20 d,其中对照组注射生理盐水0.5 mg/(kg·d),甲泼尼龙治疗组注射甲泼尼龙0.5 mg/(kg·d),IL-1RA治疗组皮下注射IL-lRA 100 pg/只。观察各组大鼠外观、测量血气,进行肺组织病理学检查并计算平均肺泡数(MAN)和肺平均内衬间隔(MLI);采用酶联免疫吸附试验检测各组大鼠支气管肺泡灌洗液(BALF)中高迁移率族蛋白B1(HMGB1)含量;采用Western blot法检测各组大鼠肺组织中HMGB1的表达。结果治疗后,三组大鼠MAN、MLI比较,差异有统计学意义(P<0.05),其中IL-1RA治疗组MAN大于甲泼尼龙治疗组和对照组(P<0.05),且其MLI明显小于甲泼尼龙治疗组和对照组(P<0.05)。三组大鼠BALF和肺组织中HMGB1表达量比较,差异有统计学意义(P<0.05),其中IL-1RA治疗组BALF中HMGB1表达量低于甲泼尼龙治疗组和对照组(P<0.05);且其肺组织中HMGB1表达量低于甲泼尼龙治疗组和对照组(P<0.05)。结论IL-RA可以降低COPD大鼠模型肺组织和肺泡中HMGB1的表达,降低大鼠气道炎症,改善大鼠血气功能,对气道重塑具有保护作用。
Objective To investigate the protective effect of interleukin-1 receptor antagonist(IL-1RA)on airway inflammation and airway remodeling in rats with chronic obstructive pulmonary disease(COPD).Methods Thirty Wistar rats were induced by smoking and intratracheal injection of lipopolysaccharide(LPS).They were divided into control group,methylprednisolone treatment group and IL-1RA treatment group according to random number table method,with 10 rats in each group.Rats in each group were continuously intervened for 20 days starting on the 20th day.The control group was injected with 0.5 mg/(kg·d)saline,the methylprednisolone treatment group was injected with 0.5 mg/(kg·d)methylprednisolone,and the IL-1RA treatment group was subcutaneously injected with IL-lRA l00 pg per rat.The appearance of rats in each group was observed,blood gas was measured,lung histopathological examination was carried out,and mean alveolar number(MAN)and mean lining interval(MLI)were calculated.The content of High mobility group protein B1(HMGB1)in bronchoalveolar lavage fluid(BALF)of rats in each group was detected by enzyme-linked immunosorbent assay,and the expression of HMGB1 in lung tissue of rats in each group was detected by Western blot.Results After treatment,there were significant differences in MAN and MLI among the three groups(P<0.05).MAN in IL-1RA group was higher than that in methylprednisolone group and control group(P<0.05),and MLI in IL-1RA group was significantly lower than that in methylprednisolone group and control group(P<0.05).The expression of HMGB1 in BALF and lung tissue of three groups was significantly different(P<0.05).The expression of HMGB1 in BALF of IL-1RA group was lower than that of methylprednisolone group and control group(P<0.05),and the expression of HMGB1 in lung tissue was lower than that of methylprednisolone group and control group(P<0.05).Conclusion IL-RA can reduce the expression of HMGB1 in lung tissue and alveoli of COPD rats,reduce airway inflammation,improve blood gas function and protect airway remodeling.
作者
刘菁
魏风芹
赵伟业
栾念旭
刘学东
LIU Jing;WEI Fengqin;ZHAO Weiye;LUAN Nianxu;LIU Xuedong(Department of Respiration,Qingdao Municipal Hospital,Shandong Province,Qingdao266012,China)
出处
《中国医药导报》
CAS
2019年第23期21-25,共5页
China Medical Herald
基金
山东省青岛市科技局面上项目(2014Y001)
关键词
慢性阻塞性肺疾病
白细胞介素1受体拮抗剂
高迁移率族蛋白B1
炎症
气道重塑
Chronic obstructive pulmonary disease
Interleukin-1 receptor antagonist
High mobility group protein B1
Inflammation
Airway remodeling
作者简介
通讯作者:刘学东。
