摘要
目的分析新生儿溶血病(HDN)脐带血三项试验结果,结合母亲血型进行统计分析,了解HDN的检出率和血型分布。方法收集该院2 865例脐带血标本进行血型鉴定和不规则抗体筛查,对母婴血型不合的标本进行HDN三项试验(分别为直接抗人球蛋白试验、游离抗体试验及释放试验),对抗体筛查阳性的标本进行抗体鉴定和脐带血三项试验。结果2 865例脐带血标本中母婴ABO及其他血型不合1 556例,占54.3%,其中确诊HDN 987例,占63.4%。996例确诊和可疑病例中,ABO血型系统959例(96.3%),其中抗A抗体562例(56.4%)、抗B抗体388例(38.9%),母婴血型O-A、O-B分别为552例(55.4%)、385例(38.6%);其他血型系统37例(3.7%),其中抗D抗体26例(12.0%)、抗E抗体5例(2.3%)、抗Ec抗体4例(1.8%)、抗M抗体2例(0.9%)。结论HDN主要发生于ABO血型系统,其次为Rh血型系统;ABO血型系统中,HDN绝大多数见于母婴血型O-A、O-B型,且O-A型多于O-B型。
Objective To analyze the three experimental results of umbilical cord blood,and to conduct the statistical analysis by combining with maternal blood group for understanding the detection rate and blood group distribution of hemolytic disease in newborn(HDN).Methods A total of 2 865 umbilical cord blood samples conducted the blood group identification and irregular antibody screening,the samples of maternal and newborn blood group incompatibility conducted direct 3-item tests(anti-human globulin test,free antibody test and release test),the samples with antibody screening positive conducted antibody identification and umbilical blood 3-item tests.Results Among 2 865 umbilical cord blood samples,1 556 cases(54.3%)were maternal and infant ABO blood group and other group incompatibility,in which 987 cases(63.4%)were diagnosed as HDN.Among 996 definitely diagnosed and suspicious cases,959 cases(96.3%)were ABO blood group system,including 562 cases(56.4%)of anti-A antibody,388 cases(38.9%)of anti-B antibody,the maternal and infant blood groups O-A and O-B had 552 cases(55.4%)and 385 cases(38.6%)respectively,and other blood group system had 37 cases(3.7%),including 26 cases(12.0%)of anti-D antibody,5 cases(2.3%)of anti-E antibody and 4 cases(1.8%)of anti-Ec antibody.Two cases(0.9%)were anti-M antibody in the MNSs blood group system.Conclusion HDN mainly occurs in ABO blood group system,followed by Rh blood group system;in ABO blood group system,HDN mostly occurs in maternal and infant blood group O-A,O-B,moreover the O-A type is more than the O-B type.
作者
黄津明
周小兰
HUANG Jinming;ZHOU Xiaolan(Department of Clinical Laboratory,Suizhou Municipal Hospital of Traditional Chinese Medicine,Suizhou,Hubei 441300,China)
出处
《检验医学与临床》
CAS
2019年第16期2316-2318,2322,共4页
Laboratory Medicine and Clinic
关键词
脐带血
新生儿溶血病
抗人球蛋白试验
游离抗体试验
释放试验
不规则抗体
血型
umbilical cord blood
hemolytic disease of newborn
anti-human globulin test
free antibody test
release test
irregular antibody
blood group
作者简介
黄津明,女,主管技师,主要从事临床检验和临床输血相关研究;通信作者:周小兰,E-mail:45526124@qq.com
