摘要
目的该研究通过对稀释液产生的基质效应进行分析,以期为游离甲状腺素(FT4)选择适当的稀释介质、稀释方法提供依据。方法收集FT4血清标本20例,其中FT4高值组(22.7~155.0pmol/L)、FT4中值组(11.5~22.7pmol/L)标本各10例。分别使用0.9%氯化钠溶液、蒸馏水、10%牛血清+0.9%氯化钠溶液、10%牛血清+蒸馏水作为稀释介质进行手工2倍(1∶1)及4倍(1∶3)稀释后测定FT4水平,并与未稀释标本的血清FT4检测值进行比较。结果中、高值FT4标本使用0.9%氯化钠溶液或蒸馏水作为稀释液进行2倍稀释与直接测定值差异最小(P>0.05)。结论该研究推测,对于FT4超过检测上限的血清标本可使用0.9%的氯化钠溶液或蒸馏水对标本进行2倍稀释。
Objective To analyze the matrix effect produced by diluent in order to provide a basis for choosing appropriate dilution medium and dilution mode of free thyroid hormone (FT4). Methods Twenty serum samples of FT4 were collected,of which 10 cases in the high value group (22.7-155.0 pmol/L) and 10 cases in the medium value group (11.5-22.7 pmol/L).With 0.9% sodium chloride solution,distilled water,10% bovine serum + 0.9% sodium chloride,10% bovine serum + distilled water as the dilution medium,FT4 was determined after manual dilution by 2 times (1∶1) and 4 times (1∶3),and the detected results were compared with the FT4 detected value of undiluted sample. Results The difference between the results of high value and medium value of FT4 sample which diluted by 2 times with 0.9% sodium chloride solution or distilled water as the diluent and the directly detected values was the smallest ( P >0.05). Conclusion This study speculates that for serum sample of FT4 exceeding the detection upper limit,0.9% sodium chloride solution or distilled water could be used to dilute the samples by 2 times.
作者
李娜
黎锋
薛声能
唐菊英
张小云
吴宏适
严励
LI Na;LI Feng;XUE Shengneng;TANG Juying;ZHANG Xiaoyun;WU Hongshi;YAN Li(Department of Endocrinology,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou,Guangdong 510120,China)
出处
《检验医学与临床》
CAS
2019年第16期2273-2275,共3页
Laboratory Medicine and Clinic
基金
国家自然科学基金青年科学基金项目(81500640)
关键词
游离甲状腺素
稀释试验
稀释介质
free thyroxine hormone
dilution test
dilution medium
作者简介
李娜,女,主管技师,主要从事内分泌相关激素的检验研究;通信作者:严励,E-mail:hfxyl@163.net
