摘要
槐角染料木黄酮处理大鼠小肠上皮细胞(IEC-6) 24 h后,接受X射线照射至吸收剂量6 Gy,48 h后采用Cell counting kit-8试剂盒和流式细胞术检测细胞增殖和凋亡,72 h后采用彗星电泳实验检测细胞DNA损伤程度。C57BL/6J雌性小鼠按200 mg/kg剂量颈部皮下注射槐角染料木黄酮,24 h后接受X射线全身照射至剂量7.5 Gy。8 d后HE染色观察小肠病理形态改变,采用免疫组化法检测小肠干扰素γ(IFN-γ)表达情况,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测小肠细胞凋亡情况。结果显示,低剂量槐角染料木黄酮能促进IEC-6细胞增殖,提高辐照后细胞存活率,高剂量则抑制细胞增殖,与辐射对照组相比,5μmol/L槐角染料木黄酮预处理能有效提高IEC-6细胞存活率,减少辐照诱发的细胞凋亡,并显著降低彗尾DNA含量和尾距;提前给予槐角染料木黄酮的小鼠与辐射对照组的小鼠相比,小肠绒毛脱落减少,肠道结构破坏得到改善,小肠组织中IFN-γ表达降低,细胞凋亡程度得到缓解。结果提示,槐角染料木黄酮对放射导致的肠损伤具有明显的保护作用。
This study investigated the protective effect of genistein extracted from fructus sophorae(FSGen) on intestinal damage caused by radiation. After FSGen treatment of rat intestinal epithelial cells(IEC-6) for 24 h, the cells were irradiated with X-rays at an absorbed dose of 6 Gy. Cell counting kit-8 and flow cytometry were used to detect cell proliferation and apoptosis 48 h after irradiation, and the extent of DNA damage was detected by comet assay 72 h later. C57 BL/6 J female mice were subcutaneously injected with FSGen at a dose of 200 mg/kg. After 24 h, they received whole-body irradiation with X-rays at a dose of 7.5 Gy. After 8 d, pathological changes in the small intestine were observed by HE staining. The expression of intestinal IFN-γ was detected by immunohistochemistry,and the apoptosis of small intestinal cells was detected by TdT-mediated dUTP nick-end labeling. Results showed that a low dose of FSgen could promote the proliferation of IEC-6 cells and improve survival rate after irradiation,whereas a high dose can inhibit cell proliferation. Compared with the radiation control group, 5 μmol/L FSGen pretreatment could effectively improve the survival rate of IEC-6 cells, reduce the apoptosis induced by irradiation,and significantly reduce the comet tail DNA content and tail moment. Compared with the radiation control group,the mice administered with FSGen in advance had less villus shedding, better intestinal structure damage, lower expression of IFN-γ in small intestine tissue, and less apoptosis. The results reveal that FSGen has a significant protective effect on radiation-induced intestinal injury.
作者
逄志骏
王昭维
徐传洋
张旭涛
何磊
李萌
张英起
李正民
PANG Zhijun;WANG Zhaowei;XU Chuanyang;ZHANG Xutao;HE Lei;LI Meng;ZHANG Yingqi;LI Zhengmin(Department of Biopharmaceutics,School of Pharmacy,The Fourth Military Medical University,Xi’an 710032,China;Clinical Laboratory,971 Hospital of Navy,Qingdao 266000,China;Department of Anesthesiology,Tangdu Hospital,The Fourth Military Medical University,Xi’an 710038,China)
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2019年第1期36-43,共8页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金项目(81673020)资助~~
关键词
辐射损伤
染料木黄酮
辐射防护剂
槐角
Radiation injury
Genistein
Radiation-protective agents
Fructus sophorae
作者简介
第一作者:逄志骏,男,1990年5月出生,2012年6月于第二军医大学获理学学士学位,现为第四军医大学药学系硕士研究生,研究方向为生物化学与分子生物学,E-mail:bigpie@yeah.net;通信作者:李正民,主治医师,E-mail:lizhengminmazui@163.com
