摘要
为探讨混播比例和刈割茬次对一年生禾豆混播草地土壤细菌群落的影响,本研究设多花黑麦草(Lolium multiflorum Lamk.)100%(R 1)、75%多花黑麦草+25%箭筈豌豆(Vicia sativa L.)(R 2)、50%多花黑麦草+50%箭筈豌豆(R 3)、25%多花黑麦草+75%箭筈豌豆(R 4)与100%箭筈豌豆(R 5)5个处理,分别于2017年1月4日(H 1)、3月14日(H 2)、4月21日(H 3)和5月19日(H 4)4个时期刈割,并采集0~20 cm土壤样本,分析土壤细菌群落组成。结果表明,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、酸杆菌门(Acidobacteria)、芽单胞菌门(Gemmatimonadetes)、硝化螺旋菌门(Nitrospirae)、浮霉菌门(Planctomycetes)、绿弯菌门(Chloroflexi)和疣微菌门(Verrucomicrobia)是混播草地主要土壤细菌群落。禾豆混播比例对土壤alpha多样性无显著性影响,刈割茬次对土壤细菌操作分类单元(Operational Taxonomic Unit,OTU)和物种丰富度指标(Chao 1指数)影响效果显著(P<0.05),改变了细菌群落结构。刈割茬次引起土壤细菌群落变化,影响了多花黑麦草和箭筈豌豆混播草地土壤细菌群落结构的均匀性。
This study was aimed to determine the effects of grass/legume ratio and cutting time on soil bacterial community of grass-legume mixture grassland.Fifteen plots were sowed in September 2016,with five treatments(100%annual ryegrass(Lolium multiflorum Lam.)(R 1),75%annual ryegrass+25%common vetch(Vicia sativa L.)(R 2),50%annual ryegrass+50%common vetch(R 3),25%annual ryegrass+75%common vetch(R 4)and 100%common vetch(R 5)).Samples of 0~20 cm soils from each plot in 2017 were collected after mixture forages harvested at H 1(January 4),H 2(March 14),H 3(April 21)and H 4(May 19),respectively.Proteobacteria),Actinobacteria,Firmicutes,Bacteroidetes,Acidobacteria,Gemmatimonadetes,Nitrospirae,Planctomycetes and Verrucomicrobia were the main soil bacterial phyla in annual ryegrass and common vetch mixtures.Ratio of grass/legume had no effects on soil bacterial alpha diversity,Cutting time significantly affected soil bacteria OTU and Chao 1 index(P<0.05),changed the bacterial community structure.Thus,cutting time could induce changes in soil bacterial community and influence the uniformity of the bacterial community structure in annual ryegrass and common vetch mixture.
作者
苟文龙
李平
董臣飞
李达旭
白史且
师尚礼
GOU Wen-long;LI Ping;Dong Chen-fei;Li Da-xu;BAI Shi-qie;SHI Shang-li(College of Pratacultura Science,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China;Sichuan Academy of Grassland Sciences,Chengdu,Sichuan Province 611731,China;Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu Province 210014,China)
出处
《草地学报》
CAS
CSCD
北大核心
2019年第5期1222-1230,共9页
Acta Agrestia Sinica
基金
四川省重点研发面上项目（2019YFN0137）
国家重点研发项目（2017YFD0502）资助
关键词
多花黑麦草
箭筈豌豆
混播比例
刈割茬次
土壤
细菌群落
Annual ryegrass
Common vetch
Mixture sowing ratio
Cutting time
Soil
Bacterial community
作者简介
苟文龙(1975-),男,陕西咸阳人,副研究员,博士研究生,主要从事牧草栽培研究,E-mail:514240346@qq.com;通信作者:师尚礼,E-mail:shishl@gsau.edu.cn.
