摘要
骨组织工程是目前治疗大段骨缺损措施中最有发展前景的途径之一,体外构建骨组织工程移植体时常采用生物反应器对细胞-支架复合体进行灌流培养,以获得具有良好修复效果的骨组织工程移植体。但目前对这一过程中种子细胞(特别是干细胞)生长率的理论建模还不完善,尤其是忽略了干细胞与终末细胞的差异。在本实验室前期研究基础上,本文利用实验室自制的灌流装置对细胞支架复合体施加不同模式和大小的流体剪切力刺激,考察流体剪切力对间充质干细胞(MSCs)增殖和成骨分化的影响,建立了流体剪切力作用对MSCs单个细胞生长率影响的回归分析模型。结果表明,0.022 5 Pa振荡剪切力更能促进MSCs增殖和成骨分化,同时修正了流体剪切力作用下MSCs单个细胞生长率的理论模型。基于以上结果,希望本文研究可为骨组织工程移植体体外灌流培养条件的优化提供理论指导。
Bone tissue engineering is considered as one of the most promising way to treat large segmental bone defect. When constructing bone tissue engineering graft in vitro, suitable bioreactor is usually used to incubate cellscaffold complex under perfusion to obtain bone tissue engineering graft with good repair efficiency. However, the theoretical model for growth rate of single cell(especially for stem cell) during this process still has many defects. The difference between stem cells and terminally differentiated cells is always ignored. Based on our previous studies, this study used self-made perfusion apparatus to apply different modes and strengths of fluid shear stress(FSS) to the cells seeded on scaffolds. The effects of FSS on the proliferation and osteogenic differentiation of mesenchymal stem cells(MSCs) were investigated. The regression analysis model of the effect of FSS on the single-cell growth rate of MSCs was further established. The results showed that 0.022 5 Pa oscillatory shear stress had stronger ability to promote proliferation and osteogenic differentiation of MSCs, and the growth rate of a single MSC cell under FSS was modified. This study is expected to provide theoretical guidance for optimizing the perfusion culture condition of bone tissue engineering grafts in vitro.
作者
李强
杨力
吕永钢
LI Qiang;YANG Li;LU Yonggang(Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqing 400044, P.R.China;Bioengineering College, Chongqing University, Chongqing 400044, P.R.China)
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2019年第5期795-802,共8页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(11702043,11872134)
关键词
间充质干细胞
流体剪切力
生物反应器
细胞生长率
成骨分化
mesenchymal stem cells
fluid shear stress
bioreactor
cell growth rate
osteogenic differentiation
作者简介
通信作者:吕永钢,Email:yglv@cqu.edu.cn.
