摘要
研究深裂竹根七中酚类化合物的种类与含量。采用福林酚法测定总酚酸含量,采用反相高效液相色谱与电喷雾电离飞行时间质谱分离鉴定酚类化合物中的4种成分,并用超高效液相色谱定量分析。乙酸乙酯提取物中总酚酸含量为(35.3±1.03)mg/g,提取物中芦丁、木犀草素、槲皮素和桦木酸4种酚酸成分含量分别为(5.63±0.15)、(2.51±0.10)、(3.87±0.12)、(2.41±0.09)μg/g。深裂竹根七乙酸乙酯提取物中含有芦丁、木犀草素、槲皮素和桦木酸4种酚酸成分。所建立的方法能有效的进行深裂竹根七中这4种酚酸的定性和定量分析。
The qualitative and quantitative analysis of phenolic compounds in Disporopsis pernyi (Hua) Diels was described.The Folin method was used to determine the total phenols content in extraction.Polyphenols were identified by electrospray ionisation time-of-flight mass spectrometry(ESI-TOF-MS) and quantified by ultra performance liquid chromatography (UPLC).The content of total phenols was (35.3±1.03) mg/g in ethyl acetate extraction (EAE).A total of 4 polyphenolic compounds found in extract were identified as rutin, luteolin, quercetin, and betulinic acid, and their contents in the extrat were (5.63±0.15),(2.51±0.10),(3.87±0.12),(2.41±0.09)μg/g respectively.The ethyl acetate extract of Disporopsis pernyi contained rutin, luteolin, quercetin, and betulinic acid.The methods could effectively perform the qualitative and quantitative analysis of phenolic compounds in Disporopsis pernyi.
作者
李开金
冯婷婷
黄晔
林奇泗
LI Kai-jin;FENG Ting-ting;HUANG Ye;LIN Qi-si(College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China)
出处
《食品研究与开发》
CAS
北大核心
2019年第18期183-187,共5页
Food Research and Development
基金
2017年徐州医科大学江苏省高校品牌建设专项课题(Xjyppzx201702)
关键词
深裂竹根七
酚类化合物
电喷雾飞行时间质谱法
超高效液相色谱
Disporopsis pernyi
phenolic
electrospray ionisation time-of-flight mass spectrometry
ultra performance liquid chromatography
作者简介
李开金(1992-),男(汉),硕士研究生,研究方向:中西药质量控制技术;通信作者:林奇泗(1980-),男(汉),副教授,硕士生导师,主要从事中西药质量控制技术研究。
