摘要
目的:探讨砷染毒对大鼠睾丸凋亡诱导因子(AIF)表达及生精细胞凋亡的影响.方法:雄性SD大鼠被随机分为对照组和染砷组.采用自由饮用方式进行连续染毒6个月.采用免疫印迹及实时荧光定量PCR检测AIF表达水平;采用TUNEL法观察生精细胞凋亡的情况.结果:免疫印迹及实时荧光定量PCR结果显示,与对照组比较,染砷组大鼠睾丸内AIF蛋白及mRNA表达水平均显著增高.TUNEL法检测结果显示与对照组比较,染砷组生精上皮凋亡细胞平均灰度值显著增高.结论:砷染毒时AIF介导的非caspase依赖性凋亡途径可能参与了大鼠生精细胞凋亡.
Objective :To investigate the effect of arsenic poisoning on the level of apoptosis inducing factor (AIF) expression and the apoptosis of spermatogenic cells in the testis of rats. Methods :Male SD rats were randomly divided into control group(distilled water) and 60.0 mg/L sodium arsenite groups,with 20 rats in each group. The rats were exposed to sodium arsenite by free drinking for six consecutive months. The protein and mRNA expression of AIF were observed by Western blotting and RT-PCR,the apoptosis of spermatogenic cells was observed by TUNEL method. Results :The results of Western blotting and RT-PCR showed that compared with control group,the protein and mRNA expression levels of AIF in the testis of the arsenic infected group were higher,and the difference was statistically significant. The results of TUNEL method showed that compared with the control group,the mean gray value of spermatogenic epithelial apoptosis cells was increased in the arsenic infected group. Conclusion : The AIFmediated non-caspase-dependent way may be involved in spermatogenic cell apoptosis in the testis of rats.
作者
戴研平
高晓勤
Dai Yanping;Gao Xiaoqin(Department of Basic Medical College,Guizhou Medical University,Guiyang 550004;Department of Basic Medical College,Zunyi Medical and Pharmaceulical College,Zunyi 563006;People's Hospital in Yueyanglou District,Yueyang 414000,China)
出处
《解剖学杂志》
CAS
2019年第4期354-357,共4页
Chinese Journal of Anatomy
基金
贵州省科学技术基金（黔科合人才团队[2014]4005号）
贵州省科学技术基金（黔科合LH字）[2015]7568号）
遵义市科技计划（遵市科合设字[2014]01号）
关键词
砷染毒
凋亡诱导因子
生精细胞
凋亡
大鼠
arsenic poisoning
apoptosis inducing factor
spermatogenic cell
apoptosis
rat
作者简介
第一作者:戴研平,E-mail:343620073@qq.com;通信作者:高晓勤,E-mail:gxq550301@sina.com.
