摘要
跳钩虾Platorchestia japonica栖息于湖泊、河流岸边,是重要的环境指示生物。本研究以线粒体COⅠ基因片段为分子标记,对北京及其周边地区22个采样点的128个样本进行种群遗传多样性和遗传结构研究。结果显示,623 bp的COⅠ基因序列中有567个保守位点、56个变异位点和38个简约信息位点。128个样本检测到43个单倍型,单倍型多样性为0.938,核苷酸多样性为0.011 72。最大似然法和贝叶斯法构建的系统发育树以及单倍型网络图表明,研究区域跳钩虾没有明显的地理种群结构,但所有单倍型形成2个遗传进化支。分子变异分析结果证实,跳钩虾2个进化支间的遗传变异显著高于进化支内的,进化支间固定系数为0.852 19,表明2个进化支遗传分化明显。本研究为进一步研究中国区域跳钩虾的遗传结构提供了有意义的基础数据。
Crustaceans of Platorchestia japonica have been investigated for their suitability to serve as indicators for the quality of water bodies,since they normally live along rivers and lakes.This study intends to explore the population genetics of P.japonica around Beijing and its adjacent regions.The mitochondrial COⅠgenes of 128 P.japonica individuals collected from 22 sampling sites were sequenced.Polymorphism analysis of P.japonica COⅠgene showed that there were 567 conserved sites,56 variable sites,and 38 parsimony informative sites.Genetic diversity analysis discovered 43 haplotypes,with haplotype diversity of 0.938 and nucleotide diversity of 0.011 72.Phylogenetic analyses using Maximum Likelihood,Bayesian methods and network analyses revealed that the haplotypes of P.japonica showed no apparent phylogeographic structure,but all haplotypes clustered into 2 clades.The results of molecular variance analyses approved that the genetic variation among the clades of P.japonica was significantly higher than that within clades,with a F st value of 0.852 19.These findings provide a scientific basis for further studying the genetic structure of P.japonica in China.
作者
崔昕宇
刘宏广
李枢强
CUI Xinyu;LIU Hongguang;LI Shuqiang(The High School Affiliated to Renmin University of China,Beijing 100080,China;Key Laboratory of ZoologicalSystematics and Evolution,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China)
出处
《四川动物》
北大核心
2019年第4期402-407,共6页
Sichuan Journal of Zoology
基金
国家自然科学基金项目(31772417)
关键词
跳钩虾
COⅠ
系统发育
遗传结构
遗传多样性
Platorchestia japonica
COⅠ
phylogeny
genetic structure
genetic diversity
作者简介
崔昕宇(2003—),女,高中生;通信作者:李枢强,E-mail:lisq@ioz.ac.cn.
