摘要
为探究大豆苗期干旱外源褪黑素对其抗氧化性和产量的影响,以干旱敏感型大豆品种绥农26为试验材料,分析水分胁迫下叶面喷施和根施褪黑素对大豆叶片和根系抗氧化酶(SOD、POD、CAT)活性和丙二醛(MDA)含量以及产量的影响。结果表明:与水分胁迫(CK)相比,施用褪黑素显著提高大豆叶片抗氧化酶活性,处理后第4天,叶面喷施和根施处理与CK相比,SOD活性在V5期分别提高6.3%和19.5%,POD、CAT活性在V1期分别提高7.5%、12.7%和5.6%、9.9%(P<0.05),MDA含量在V3期分别降低了9.4%、10.3%(P<0.05);处理后第7天,叶面喷施褪黑素与对照相比,在V1期SOD、POD、CAT活性分别提高了6.3%、9.5%、4.9%(P<0.05),而根施褪黑素处理较对照分别提高了6.9%、20.9%、11.1%(P<0.05),MDA含量叶面喷施和根施褪黑素处理与CK相比分别降低了6.9%、10.3%(P<0.05);V1、V3和V5期叶面喷施褪黑素处理的产量与CK相比分别提高了31.6%、29.8%、12.6%(P<0.05),而根施褪黑素处理的产量较对照分别提高了43.3%、31.8%、24.9%(P<0.05)。结果表明:外源褪黑素通过降低MDA含量,提高抗氧化酶活性、维持细胞水分平衡、提高大豆的抗旱能力,最终提高其产量,而且根施褪黑素效果要显著优于叶施。
To explore the effects of exogenous melatonin on the antioxidant properties and yield of soybean on its seedling drought, we used soybean[Glycine max(L.) Merrill] cultivar Suinong 26 with drought sensitive, this study investigated the effects of antioxidant enzymes activity(SOD, POD, CAT), malondialdehyde(MDA) content and yield of soybean leaves and roots under water stress by using melatonin on the leaf and root. Our results indicated that compared with water stress(CK), the antioxidant enzyme activity of soybean leaves was significantly increased by using melatonin. When applyed melatonin on the leaf and root at 4 d after treatment, the activity of SOD was increased by 6.3% and 19.5% at V5 stage(P<0.05), POD increased by 7.5% and 12.7%(P<0.05), CAT increased by 5.6% and 9.9% at V1 stage(P<0.05), and the contents of MDA decreased by 9.4% and 10.3% at V3 stage(P<0.05) as compared to the CK. When compared to the CK at the 7 d after treatment, the activity of SOD, POD, CAT with applying melatonin of leaf at V1 stage increased by 6.3%, 9.5% and 4.9%, respectively. The activity of SOD, POD, CAT with applying melatonin of root increased by 6.9%, 20.9% and 11.1%, respectively. The content of MDA with applying melatonin of leaf and root at V1 stage decreased by 6.9% and 10.3%(P<0.05) at V1 stage. Compared to the CK, yield of applying melatonin of leaf at V1, V3 and V5 stages were increased by 31.6%, 29.8% and 12.6%, respectively, and yield of applying melatonin on root at V1, V3 and V5 stages were increased by 43.3%, 31.8% and 24.9%, respectively. The result showed that exogenous melatonin could improve the drought resistance of soybean, increased the yield and maintained the water balance of the cells by means of reducing the content of malondialdehyde, and finally increased the activity of antioxidant enzymes. Furthermore, the root application of melatonin was better than leaf application.
作者
何松榆
秦彬
张明聪
金喜军
王孟雪
任春元
张玉先
HE Song-yu;QIN Bin;ZHANG Ming-cong;JIN Xi-jun;WANG Meng-xue;REN Chun-yuan;ZHANG Yu-xian(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2019年第3期407-412,共6页
Soybean Science
基金
国家自然科学基金(31801303)
黑龙江省自然科学基金面上项目(C2016042)
中国博士后科学基金(2016M591568)
黑龙江八一农垦大学博士科研启动基金(XYB2014-04)
国家现代农业产业技术体系(CARS-04-PS17)
关键词
大豆
苗期
褪黑素
水分胁迫
抗氧化酶
产量
Soybean
Seedling stage
Melatonin
Water stress
Antioxidant enzymes
Yield
作者简介
第一作者:何松榆(1992-),男,硕士,主要从事大豆高产生理生态研究。E-mail:hsyspark@163.com;通讯作者:张玉先(1968-),男,博士,教授,博导,主要从事大豆高产生理生态研究。E-mail:zyx_lxy@126.com。
