摘要
目的:探讨芪术方(QZP)诱导人肝癌细胞SMMC-7721凋亡的分子机制。方法:以不同浓度的QZP干预人肝癌细胞SMMC-7721,流式细胞仪检测细胞凋亡及周期分布情况,RT-PCR法检测AKT1、AKT2、Caspase-9mRNA的表达;蛋白质印迹法检测p-AKT、PTEN蛋白表达。结果:QZP能阻滞人肝癌细胞SMMC-7721从G0/G1期进入S期,并诱发细胞凋亡;能抑制AKT1、AKT2表达,提高Caspase-9表达,且跟浓度呈正比例关系。结论:QZP能诱导人肝癌细胞SMMC-7721凋亡和阻滞细胞周期,其机制可能为促进人肝癌细胞SMMC-7721 PTEN表达的增加,使得PI3K/AKT信号通路激活受阻,同时抑制p-AKT、AKT1、AKT2的表达,激活下游Caspase-9促凋亡分子的表达,从而为PI3K/AKT信号通路介导细胞凋亡这一设想提供了理论依据。
Objective: To discuss molecular mechanism of QiZhu prescription (QZP) inducing the apoptosis of human liver cancer cells SMMC-7721. Methods: Different concentrations of QZP were used to intervene human liver cancer cells SMMC-7721, flow cytometry was used to detect cellular apoptosis and cycle distribution conditions,RT-PCR was adopted to detect the expressions of AKT1, AKT2 and Caspase-9mRNA, Western blotting was used to detect the expressions of p-AKT and PTEN protein. Results: QZP could arrest human liver cancer cells SMMC-7721 transiting from G0/G1 phase to S phase, induce cellular apoptosis;it could inhibit the expressions of AKT1 and AKT2, raise the expression of caspase-9, and the effects of QZP varies directly with the concentrations. Conclusion: QZP could induce the apoptosis of human liver cancer cell SMMC-7221 and arrest cellular cycle, and it might increase the expressions of human liver cancer cells SMMC-7721 PTEN, block the activation of PI3K/AKT signal channel, inhibit the expressions of p-AKT, AKT1 and AKT2, and activate the expressions of caspase-9, therefore to provide theoretical evidencefor PI3K/AKTsignal channelmediatingcellular apoptosis.
作者
赵冬耕
凌昌全
ZHAO Donggeng;LING Changquan(Shanghai University of TCM, Shanghai 201203, China;Nantong Hospital of Traditional Chinese Medicine;Changhai Hospital Affiliated to the Second Military Medical University)
出处
《西部中医药》
2019年第3期44-48,共5页
Western Journal of Traditional Chinese Medicine
基金
上海市中医药事业发展三年行动计划(编号ZY3-LCPT-2-1004)
长海医院1255学科建设计划课题(编号CH125521200)
上海市科学技术委员会医学引导项目(编号15401931700)
作者简介
赵冬耕(1985-),男,在读博士研究生,主治医师。研究方向:肿瘤的中西医结合治疗;通讯作者:凌昌全(1957-),博士研究生导师,博士学位,教授,主任医师。研究方向:肝癌的中医药防治。
