摘要
本文以分离自波兰开菲尔粒的开菲尔乳杆菌(Lactobacillus kefiri,L.kefiri)KL22为研究对象,研究了其生长和产酸曲线,并通过分析其疏水性、自凝聚力、降胆固醇能力和对人源肠细胞Caco-2的免疫影响,来评价其益生特性。结果表明,KL22对二甲苯、三氯甲烷和乙酸乙酯的疏水性均大于80%,放置5 h的自凝聚力为21.17%;具有降胆固醇能力,培养48 h后,活性KL22的胆固醇降解率为52.94%;对Caco-2细胞中白细胞介素8(Interleukin-8,IL-8)、肿瘤坏死因子(Tumor necrosis factor-α,TNF-α)和β防御素2(β-Defensin 2,BD-2)的表达水平具有调控作用,当菌液浓度为10~8 CFU/mL时, IL-8和TNF-α的表达量上调,而BD-2的表达量下调;当菌液浓度为10~6 CFU/mL时,IL-8、TNF-α和BD-2的表达量均下调。在抗生素敏感性方面,菌株对四环素表现为中度敏感,对环丙沙星、万古霉素、复方新诺明和苯唑西林表现为抗性,对其它25种抗生素表现为敏感(S)。综上所述,该菌株可作为潜在的益生性菌株。
The article was based on Lactobacillus kefiri KL22 which was isolated from Polish Kefir.The rate of growth and acid producting were tested firstly.Then the certain probiotic properties of KL22 were evaluated,which contained hydrophobicity,self-aggregation,cholesterol lowering ability,immune effects of human intestinal cells Caco-2 and antibiotics sensitivity.The results showed that the hydrophobicity of KL22 was more than 80%against xylene,chloroform or ethyl acetate.Its self-aggregation was 21.17%after cultivate 5 h in room temperature.And after co-incubated with KL22 in 48 h,the degradation of cholesterol in culture medium was 52.94%.The real-time q-PCR showed that KL22 had a regulating effect on the expression levels of IL-8,TNF-αand BD-2.When the concentration of KL22 was 108 CFU/mL,the expression levels of IL-8 and TNF-αwere up-regulated,while the expression levels of BD-2 was down-regulated.When the concentration of KL22 was 106 CFU/mL,the expression levels of IL-8、TNF-αand BD-2 were down-regulated.Finally,in aspect of antibiotic sensitivity,KL22 was moderately sensitive to tetracycline and was resistant to ciprofloxacin,vancomycin,compound sulfamethoxazole and oxacillin.In conclusion,KL22 was a potential strains which could be used as probiotic.
作者
赵彤
王永霞
荀一萍
艾连中
赵林森
王立峰
朱宏
王世杰
ZHAO Tong;WANG Yong-xia;XUN Yi-ping;AI Lian-zhong;ZHAO Lin-sen;WANG Li-feng;ZHU Hong;WANG Shi-jie(Hebei University of Engineering,Handan 056001,China;Shijiazhuang Junlebao Dairy Co.,Ltd.,Shijiazhuang 050221,China;University of Shanghai for Science and Technology,Shanghai 200093,China;Hebei Yiran Biotechnology Co.,Ltd.,Shijiazhuang 050800,China;Nanjing University Of Finance & Economics,Nanjing 210046,China)
出处
《食品工业科技》
CAS
北大核心
2019年第9期115-120,共6页
Science and Technology of Food Industry
基金
十三五国家重点研发计划(2016YFD0400605)
河北省重点研发计划(18227124D)
石家庄市科学技术研究与发展计划项目(176170877A)
关键词
开菲尔乳杆菌
益生特性
疏水性
自凝聚力
降胆固醇
Lactobacillus kefiri
probiotic properties
hydrophobicity
self-aggregation
cholesterol lowering ability
作者简介
赵彤(1994-),女,硕士研究生,研究方向:食品加工与安全,E-mail:m13583224087_1@163.com;通讯作者:朱宏(1964-),男,博士,正高级工程师,研究方向:动物食品研究,E-mail:zhuhong@jlbry.com;通讯作者:王世杰(1980-),男,博士,正高级工程师,研究方向:乳品科学,E-mail:wangshijie@jlbry.com。
