摘要
目的了解解放军总医院沙门菌流行情况及耐药基因特征,为沙门菌的感染防治提供依据。方法回顾性研究,收集78株2015至2017年沙门菌临床分离株,患者年龄(49±21)岁,以中青年为主,临床样本主要来自粪便和静脉血,分别占44.87%(35/78)和33.33%(26/78)。对其进行血清型鉴定、体外药敏试验和全基因组测序后,进行多位点序列分型(MLST)和携带耐药基因分型,对耐头孢噻肟或环丙沙星沙门菌聚类分析。结果沙门菌血清型以D群沙门菌(53.85%)和C群沙门菌(21.79%)为主;ST分型以ST11型为主。体外药敏试验显示沙门菌多药耐药率为64.11%(50/78),全部敏感率为25.64%(20/78)。对萘啶酸耐药率最高,为65.38%(51/78)。沙门菌最常携带的耐药基因为β内酰胺类药物耐药基因,占78.21%(61/78)。结论本院分离的沙门菌ST型与携带耐药基因呈多样性,多数沙门菌对抗菌药物呈现多药耐药。对沙门菌进行分子分型、耐药基因分析及构建耐药菌进化树对确定耐药沙门菌间的亲缘关系,具有一定的临床意义。
Objective To understand the prevalence of Salmonella and the characteristics of drug resistance genes in General Hospital of People′s Liberation Army,and to provide evidence for the prevention and treatment of Salmonella infection.Methods A retrospective study was conducted to collect 78 clinical isolates of Salmonella from 2015 to 2017.The age of the patients was 49±21 years old.The infected patients were mainly young and middle-aged.The clinical samples mainly came from feces and venous blood,accounting for 44.87%(35/78) and 33.33%(26/78),respectively.After serotype identification,drug sensitivity test and whole genome sequencing,multilocus sequence typing and drug resistance genotyping were performed.Cluster of Cefotaxime or Ciprofloxacin resistant Salmonella was analyzed.Result sSalmonella group D (53.85%) and Salmonella group C (21.79%) were dominant Salmonella serotype.ST11 was mainly ST type.Drug sensitivity test showed that the multidrug resistance rate of Salmonella was 64.11%(50/78).The sensitivity to all antimicrobial agents′ rate was 25.64 (20/78).The resistance rate of Salmonella to nalidixic acid was 65.38%(51/78).The most common drug resistance gene of Salmonella was extended-spectrum β-lactam drug resistance gene,accounting for 78.21%(61/78).Conclusion sThe ST-type and carrying resistance genes of Salmonella in this hospital were diverse.Most pathogens were multi-drug resistant to antimicrobial agents.Molecular typing and drug resistance gene analysis of Salmonella and construction of resistant strains to determine the inheritance of Salmonella relationships have a certain clinical significance.
作者
荆颖
罗燕萍
叶坤
李鑫
叶丽艳
张樱
何赏
王成彬
Jing Ying;Luo Yanping;Ye Kun;Li Xin;Ye Liyan;Zhang Ying;He Shang;Wang Chengbin(Clinical Laboratory Diagnostics,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou 325035,China;Medical Laboratory Center,General Hospital of People's Liberation Army,Beijing 100085,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2019年第3期209-213,共5页
Chinese Journal of Laboratory Medicine
关键词
沙门菌属
抗药性
细菌
多位点测序分型
Salmonella
Drug resistance, bacterial
Multilocus sequence typing
作者简介
通信作者:王成彬,Email:wangcb301@126.com.
