摘要
[目的]以提取率为主要评价指标,探讨咪唑类离子液体提取桤叶棠棣总黄酮的最佳工艺。[方法]通过对比不同种类、浓度的提取溶剂和不同提取方法初步确定提取工艺,并应用单因素试验和响应面Box-Behnken试验对关键因素进行优化。[结果]选取0.7 mol/L的溴化1-丁基-3-甲基咪唑水溶液为提取溶剂,其提取效率高于其他离子液体及70%甲醇、70%乙醇等传统有机溶剂;微波辅助法相比热回流法和超声辅助法具有显著优越性,提取得率高且可重复性好。在微波提取工艺的响应面试验中,液料比对桤叶棠棣总黄酮提取率的影响最为显著,最佳工艺条件:提取温度72℃、提取时间16.5 min、液料比24.5(mL∶g),在此条件下平均得率达12.75 mg/g。[结论]咪唑类离子液体在微波环境下能够有效提高桤叶棠棣总黄酮提取率,优化后的工艺参数可为其工业化生产和科学研究提供理论依据。
[Objective] The extraction rate was the main evaluation index, and the optimal extraction process for total flavonoids of Amelanchier alnifolia Nutt. was studied.[Method] The initial extraction process was determined by comparing different extraction methods and extraction solvents of different types and concentrations. The key factors were optimized by single factor tests and Box-Behnken tests.[Result] 0.7 mol/L 1-butyl-3-methylimidazolium bromide was selected as the extraction solvent, and the extraction efficiency was higher than other ionic liquids, 70% methanol, 70% ethanol and other traditional organic solvents;compared with the thermal reflux method and the ultrasonic-assisted method, the microwave-assisted method has significant superiority, such as high extraction yield and good repeatability. In the response surface test of microwave-assisted extraction process, material-liquid ratio had the most significant effect on the extraction rate of total flavonoids of Amelanchier alnifolia Nutt., and the optimum conditions were as follows: extraction temperature 72 ℃, extraction time 16.5 min, liquid-solid ratio 24.5 mL/g, the average yield in repeated extraction of three times could reach 12.75 mg/g.[Conclusion] The imidazole ionic liquid under microwave condition can effectively improve the extraction rate of total flavonoids from Amelanchier alnifolia Nutt.. The optimized parameters in extraction process can provide corresponding theoretical basis for industrial production and scientific research.
作者
潘思源
田英姿
PAN Si-yuan;TIAN Ying-zi(College of Light Industry Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510641)
出处
《安徽农业科学》
CAS
2019年第9期159-163,181,共6页
Journal of Anhui Agricultural Sciences
基金
自治区财政林业科技专项资金项目(-0608)
关键词
桤叶棠棣
总黄酮
离子液体
提取工艺
响应面
Amelanchier alnifolia Nutt.
Total flavonoids
Ionic liquid
Extraction process
Response surface method
作者简介
潘思源(1993-),男,陕西宝鸡人,硕士研究生,研究方向:植物资源综合利用;通信作者:田英姿,教授,硕士,硕士生导师,从事植物资源综合利用与天然产物研究。
