摘要
白藜芦醇是一种具有抗氧化、抑菌、抗癌、保护心血管、延缓衰老等诸多生理作用的多酚化合物。以药食两用的桑椹为原料,在单因素试验基础上,采用中心组合法设计响应面试验优化桑椹白藜芦醇的提取工艺条件。结果表明,桑椹中白藜芦醇提取的最佳工艺参数为:以甲醇作为提取剂,料液比1∶30,提取温度27.5℃,提取时间8.0 h。通过液相色谱对桑椹中的白藜芦醇进行定性定量分析,桑椹冻干粉中白藜芦醇的提取量为315.30μg/g。
As one of the important polyphenolic compounds,resveratrol exhibits multiple bioactivity such as anti-oxidant,anti-bacterial,antitumor,cardioprotective and anti-aging.On the basis of single factor test,the extraction process of resveratrol in mulberry fruit was optimized by central composite design-response surface experiment.The parameters in the optimum extraction process were obtained by adopting methanol as the extraction agent with material-solvent ratio 1∶ 30,extraction temperature 27.5 ℃ and extraction duration 8.0 h.Qualitative and quantitative analysis by HPLC showed that the extraction yield of resveratrol from freeze-dried mulberry fruit was 315.30 μg/g.
作者
曹喜涛
赵利安
屠洁
张业顺
季更生
张国政
Cao Xitao;Zhao Li’an;Tu Jie;Zhang Yeshun;Ji Gengsheng;Zhang Guozheng(College of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;Key Laboratory of Genetic Improvement of Silkworm and Mulberry,Ministry of Agriculture and Rural Affairs,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2019年第1期108-115,共8页
ACTA SERICOLOGICA SINICA
基金
江苏科技大学大学生创新计划项目(No.201610283002-F)
江苏科技大学博士启动基金项目(No.1732921203)
中国农业科学院蚕业研究所生物技术学院院所发展基金项目(No.2014SY10
2015SY02)
镇江市社会发展指导性项目(No.FZ2018075)
关键词
白藜芦醇
桑椹
响应面法
提取条件
Resveratrol
Mulberry fruit
Response surface method(RSM)
Extraction condition
作者简介
第一作者:曹喜涛(1976-),男,博士,助理研究员。E-mail:xtcao668@just.edu.cn;通信作者:季更生,博士,副教授。E-mail:jigsheng@just.edu.cn.
