摘要
以毛建草茎段为外植体,研究激素6-BA,NAA不同配比对愈伤组织诱导的影响,同时对影响其芽分化及生根的因素进行探讨。结果表明,最适宜诱导毛建草茎段愈伤组织形成的培养基配方是MS+NAA 0.5 mg/L+6-BA 0.5 mg/L+蔗糖30 g/L+琼脂6.5 g/L;增殖培养易生根但难以分化出芽。
The effects of different proportion of 6-BA and NAA on callus induction from stem segments of Dracocephalum rupestre Hance were studied, and the factors affecting shoot proliferation and rooting were discussed. The results showed that the most suitable medium for callus formation was MS+0.5 mg/L 6-BA+0.5 mg/L NAA+30 g/L sugar+6.5 g/L gar. Proliferation culture was easy to take root, but it was difficult to differentiate buds.
作者
宗越
郭晓红
田旭平
ZONG Yue;GUO Xiaohong;TIAN Xuping(College of Forestry,Shanxi Agricultural University,Taigu 030801,China)
出处
《山西农业科学》
2019年第4期507-509,共3页
Journal of Shanxi Agricultural Sciences
关键词
毛建草
茎段
愈伤组织
组织培养
Dracocephalum rupestre Hance
stem segment
callus
tissue culture
作者简介
宗越(1994-),女,山西神池人,在读硕士,研究方向:园林植物与观赏园艺;通信作者:田旭平。
