摘要
在室温条件下,探讨不同饥饿时间对拟环纹豹蛛体内蛋白酶和脂肪酶活性的影响。结果表明:不同饥饿时间处理的拟环纹豹蛛雌、雄成蛛体内蛋白酶和脂肪酶活性均存在差异。在饥饿初期(雌蛛0～12h、雄蛛0～4h),饥饿处理对拟环纹豹蛛体内的蛋白酶活性起促进作用,雌、雄蛛的蛋白酶活性分别较未饥饿处理上升了36.8%和35.8%,差异显著;而后期起抑制作用,但差异不显著。拟环纹豹蛛雌、雄成蛛体内的脂肪酶活性,随着饥饿时间的延长均呈下降的趋势。在饥饿的初始阶段(0～4h),饥饿处理对脂肪酶活性的抑制作用较大,雌成蛛的脂肪酶活性较未饥饿处理的脂肪酶活性下降了39.6%,差异显著,雄成蛛的脂肪酶活性较未饥饿处理的脂肪酶活性下降了15.3%,差异不显著,而后期饥饿处理对拟环纹豹蛛雌、雄成蛛的脂肪酶活性影响均不明显。此外,拟环纹豹蛛雌成蛛的蛋白酶和脂肪酶活性在各相同饥饿处理时间均高于雄成蛛的蛋白酶和脂肪酶活性。
This study was conducted to investigate the effect of starvation on major digestive enzymes(protease and lipase).The results showed that the effects of different starvation phases on digestive enzyme activities of male and female adult Pardosa pseudoannulata were different.In the early stage of starvation,the protease activities in female spiders(0-12 h)and male spiders(0-4 h)were promoted by starvation treatment,which increased by 36.8% and 35.8%,respectively,compared with that in the 0 h of starvation.However the protease activities in later stage were inhibited,but the difference was not significant.Lipase activities of female and male adult spiders decreased with the time of starvation.In the initial stage of starvation(0-4 h),the starvation treatment had a great inhibitory effect.The lipase activity of female adult spiders was 39.6% lower than that treated with starvation for 0 h,with a significant difference,while the lipase activity of male adult spiders was 15.3% lower than that treated with starvation for 0 h,with no significant difference.However in the later stage of starvation treatment,little effect on the lipase activities of female and male adult spiders was observed.Treated for the same starvation time,the activities of protease and lipase of female spiders were significantly higher than those of male spiders.
作者
刘其全
邱良妙
施龙清
占志雄
LIU Qi-quan;QIU Liang-miao;SHI Long-qing;ZHAN Zhi-xiong(Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou,Fujian 350013,China;Institute of Rice Research,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)
出处
《福建农业学报》
CAS
北大核心
2018年第12期1312-1316,共5页
Fujian Journal of Agricultural Sciences
基金
国家重点研发计划项目(2017YFD0100100)
福建省自然科学基金项目(2015J01117)
福建省农业科学院科技创新团队建设项目(STIT2017-1-8)
关键词
饥饿
拟环纹豹蛛
蛋白酶
脂肪酶
活性
starvation
Pardosa pseudoannulata
protease
lipase
activity
作者简介
刘其全(1978-),男,博士,副研究员,研究方向:昆虫生态与害虫综合治理(E-mail:liuqq1221@163.com);通讯作者:占志雄(1964-),男,研究员,研究方向:昆虫生态与害虫综合治理(E-mail:zzx64@sohu.com).
