摘要
目的研究甲亢源性房颤与心房交感神经重构相关的发病机制。方法选取50只成年健康日本大耳兔,按照随机数字表法分为给药2月组(n=20)、给药4月组(n=20)、对照组(n=10)。给药2月组每日腹腔内注射甲状腺素50μg/kg,持续2个月;给药4月组每日腹腔内注射甲状腺素50μg/kg,持续4个月;对照组每日腹腔内注射等剂量生理盐水,持续4个月。制备甲亢房颤易患动物模型,比较三组基础资料、左心房有效不应期(LAERP)、房颤诱发率,以及交感神经激活和重构相关指标的表达情况。结果①实验后,给药2月组和给药4月组体重低于对照组,且给药4月组体重低于给药2月组(P <0.05);给药2月组和给药4月组心率快于对照组(P <0.05)。②给药2月组、给药4月组的LAERP均短于对照组,且给药4月组短于给药2月组(P <0.05)。③给药4月组的房颤诱发率高于给药2月组和对照组(P <0.05)。④给药2月组和给药4月组去甲肾上腺素水平高于对照组(P <0.05)。⑤给药2月组、给药4月组酪氨酸羟化酶(TH)和生长相关蛋白-43(GAP-43)的mRNA相对表达量高于对照组(P <0.05),且给药4月组GAP-43 mRNA相对表达量高于给药2月组(P <0.05)。⑥给药2月组、给药4月组TH和GAP-43蛋白相对表达量高于对照组(P <0.05),且给药4月组GAP-43蛋白相对表达量高于给药2月组(P <0.05)。结论持续高水平甲状腺素暴露下心房交感神经被过度激活、重构,其可能是甲亢源性心房颤动的发病机制之一。
Objective To study the pathogenesis of hyperthyroidism-induced atrial fibrillation and atrial sympathetic nerve remodeling.Methods According to random number table method,50 adult healthy Japanese big ear rabbits were divided into two month administration group(n=20),four month administration group(n=20)and control group(n=10).The two month administration group received intraperitoneal injection of thyroxine 50μg/kg daily for 2 months,the four month administration group received intraperitoneal injection of thyroxine 50μg/kg daily for 4 months,and the control group received intraperitoneal injection of equal dose of normal saline for 4 months.Animal models of hyperthyroidism and atrial fibrillation vulnerability were prepared.The basic data,left atrial effective refractory period(LAERP),induced rate of atrial fibrillation,and expression of related indicators of sympathetic nerve activation and remodeling were compared among the three groups.Results①After the experiment,the body weight of two month administration group and four month administration group were lower than those of control group,and the body weight of four month administration group was lower than that of two month administration group(P<0.05);the heart rate of two month administration group and four month administration group were faster than those of control group(P<0.05).②The LAERP in the two month administration group and four month administration group were shorter than those in the control group,and that in the four month administration group was shorter than that in the two month administration group(P<0.05).③The induced rate of atrial fibrillation in the four month administration group was higher than that in the two month administration group and the control group(P<0.05).④The level of norepinephrine in the two month administration group and the four month administration group were higher than those in the control group(P<0.05).⑤The relative expression of tyrosine hydroxylase(TH)and growth associated proteins-43(GAP-43)mRNA in the two month administration group and four month administration group were higher than those in the control group(P<0.05),and the relative expression of GAP-43 mRNA in the four month administration group was higher than that in the two month administration group(P<0.05).⑥The relative expression of TH and GAP-43 protein in the two month administration group and four month administration group were higher than those in the control group(P<0.05),and the relative expression of GAP-43 protein in the four month administration group was higher than that in the two month administration group(P<0.05).Conclusion Atrial sympathetic nerve is overactivated and remodeled under continuous high level of thyroxine exposure,which may be one of the pathogenesis of hyperthyroidism-induced atrial fibrillation.
作者
郑甲林
代华磊
张新金
李建美
ZHENG Jialin;DAI Hualei;ZHANG Xinjin;LI Jianmei(Department of Cardiology,the Fourth Affiliated Hospital of Kunming Medical University,Yunnan Province,Kunming 650021,China;Department of Cardiology,Cardiovascular Hospital Affiliated to Kunming Medical University,Yunnan Province,Kunming 650021,China)
出处
《中国医药导报》
CAS
2019年第5期9-13,18,I0002,共7页
China Medical Herald
基金
云南省教育厅基金资助项目(2016ZDX062)
云南省科技厅科技计划项目[2018FE001(-285)]
关键词
心房颤动
甲亢
交感神经
重构
Atrial fibrillation
Hyperthyroidism
Sympathetic nerve
Remodeling
作者简介
郑甲林(1979.11-),男,博士,副主任医师,主要从事心律失常基础与临床方面研究;通讯作者:代华磊(1983.4-),男,硕士,副主任医师,主要从事冠心病、心律失常基础与临床方面研究。
