摘要
辣椒疫霉菌(Phytophthora capsici)产生的效应因子RxLR129113在本氏烟上的功能之一是抑制BAX引起的细胞坏死,而且已经证实乙醛脱氢酶(ALDH)是RxLR129113的互作蛋白。为了探究乙醛脱氢酶(ALDH)是否影响Rx LR129113抑制BAX引起的细胞坏死,本研究利用病毒诱导的基因沉默(VIGS)技术,克隆了乙醛脱氢酶(ALDH)基因片段,插入到烟草脆裂病毒载体(TRV)中,构建了pTRV-ALDH的VIGS载体,转入农杆菌侵染本氏烟后qrt-PCR验证成功沉默了ALDH基因。在沉默ALDH基因的本氏烟上瞬时表达RxLR129113,24 h接种BAX。结果表明,在NbALDH沉默植株上,RxLR129113仍然抑制BAX引起的细胞坏死。本研究结果表明RxLR129113与ALDH互作不影响其抑制BAX引起的细胞坏死,为进一步深入开展辣椒疫霉效应因子RxLR129113功能机制研究奠定了基础。
One of the functions of the Phytophthora capsici effector RxLR129113 on N.benthamiana is to inhibit BAX-induced cell necrosis,and acetaldehyde dehydrogenase(ALDH)has been shown to be an interaction protein of RxLR129113.To investigate whether aldehyde dehydrogenase(ALDH)affects RxLR129113 inhibition of BAX-induced cell necrosis,this study cloned the aldehyde dehydrogenase(ALDH)gene fragment and inserted it into tobacco using virus-induced gene silencing(VIGS)technology.In the fragile virus vector(TRV),the VIGS vector of pTRV-ALDH was constructed,and the ALDH gene was successfully silenced by qrt-PCR after agrobacterium infecting N.benthamiana.RxLR129113 was transiently expressed on N.benthamiana with silenced ALDH gene,and BAX was inoculated 24 h.The results showed that RxLR 129113 still inhibited BAX-induced cell necrosis on NbALDH-silenced plants.The results of this study indicate that the interaction between RxLR129113 and ALDH does not affect the inhibition of BAX-induced cell necrosis,which lays a foundation for further research on the functional mechanism of P.capsici effector RxLR129113.
作者
王俪颖
梁涛
朱彤彤
马艳飞
江伟霖
冀瑞卿
张修国
WANG Li-ying;LIANG Tao;ZHU Tong-tong;MA Yan-fei;JIANG Wei-lin;Ji Rui-qing;ZHANG Xiu-guo(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi/Jilin Agricultural University, Jilin 130118, China;College of Plant Protection/Shandong Agricultural University, Key Laboratory of Vegetable Pest and Biology of Shandong Province, Tai’an 271018, China)
出处
《山东农业大学学报（自然科学版）》
北大核心
2019年第1期31-34,共4页
Journal of Shandong Agricultural University：Natural Science Edition
基金
国家大宗蔬菜产业技术体系(CARS-25-03B)
作者简介
王俪颖(1993-),女,在读硕士研究生.研究方向:植物病原真菌学和真菌资源利用. E-mail:1479779905@qq.com;通讯作者:冀瑞卿,E-mail:jiruiqingjrq@126.com;通讯作者:张修国,E-mail:Zhxg@sdau.edu.cn.
