摘要
目的:探讨MEG3在雷公藤红素诱导的肝癌HepG2细胞凋亡和细胞增殖抑制过程中的作用。方法:采用CCK-8法检测HepG2细胞增殖情况,流式细胞仪检测细胞凋亡情况,Western blot检测cleaved caspase-3、Bax和Bcl-2蛋白的表达,real-time PCR检测MEG3的表达。结果:雷公藤红素抑制HepG2细胞增殖、促进细胞凋亡并上调MEG3的表达;敲低MEG3的表达后显著逆转了雷公藤红素诱导的HepG2细胞增殖抑制和细胞凋亡。结论:雷公藤红素通过上调MEG3的表达抑制HepG2细胞增殖、促进细胞凋亡。
Objective:To explore the role of MEG3 in the HepG2 cells apoptosis and cells proliferation induced by celastrol.Methods:CCK-8 was used to detect the proliferation of HepG2 cells.Flow cytometry was used to detect HepG2 cells apoptosis.The expressions of cleaved caspase-3,Bax and Bcl-2 protein were detected by Western blot,and real-time PCR was used to detect the expression of MEG3.Results:Celastrol inhibited the proliferation of HepG2 cells and promoted cells apoptosis by up-regulating the expression of MEG3.After knocking down MEG3 with siRNA,it significantly reversed the proliferation inhibition and apoptosis of HepG2 cells induced by celastrol.Conclusion:Celastrol inhibits HepG2 cells proliferation and promotes cell apoptosis by up-regulating MEG3.
作者
陈晓雷
杨秀丽
周天竹
周梦阳
李新新
张海燕
孟欣
Chen Xiaolei;Yang Xiuli;Zhou Tianzhu;Zhou Mengyang;Li Xinxin;Zhang Haiyan;Meng Xin(Institute of Forensic and Judicial Research,Shenyang Medical College,Liaoning Shenyang 110034,China;Department of Geriatrics,the First Affiliated Hospital,China Medical University,Liaoning Shenyang 110001,China;Department of Biochemistry and Molecular Biology,College of Basic Medicine,China Medical University,Liaoning Shenyang 110122,China)
出处
《现代肿瘤医学》
CAS
2019年第5期739-742,共4页
Journal of Modern Oncology
基金
国家自然科学基金(编号:81572831)
沈阳市科技计划项目(编号:17-230-9-27)
关键词
雷公藤红素
长链非编码RNA
MEG3
增殖
凋亡
肝癌
celastrol
long non-coding RNA
MEG3
proliferation
apoptosis
hepatocellular carcinoma
作者简介
陈晓雷(1974-),男,辽宁沈阳人,讲师,硕士,主要从事法医临床学教学、司法鉴定和法医临床科研工作。E-mail:chen_xiaolei_smc@163.com.
