摘要
目的:探究棕榈酸对肝细胞内自噬和凋亡途径的影响。方法:不同浓度棕榈酸处理肝细胞并检测自噬相关蛋白的改变; CCK-8法检测不同浓度棕榈酸对肝细胞活性的影响;棕榈酸培养细胞不同时间,观察细胞数量和形态的改变;棕榈酸培养细胞8 h后用DAPI染色观察细胞核形态。结果:棕榈酸使自噬相关蛋白表达增加,但并不表现为剂量依赖性;随着棕榈酸浓度的增加,细胞活力下降;随着棕榈酸作用时间的增加,细胞数量减少;和对照组相比,棕榈酸处理组细胞核致密浓染。结论:棕榈酸能够引起肝细胞凋亡,细胞活力下降,同时通过Erk1/2通路引起肝细胞自噬增强。
Objective: To observe the effect of palmitate on autophagy and apoptosis of hepatocytes. Methods :The hepatocytes were treated with palmitic acid in different concentration,the changes of autophagy-related proteins were detected.CCK-8 assay was performed to determine the effect of palmitic acid in different dose on the activity of hepatocytes.Hepatic cells were cultured in diverse time phase to observe the changes of cell count and morphology,and also cultured with palmitic acid for 8 h and stained with DAPI to observe alteration of the nuclei. Results :Palmitic acid increased the expression of autophagy-related protein,yet the expression was not in a dose-dependent manner.Hepatic cell activity was decreased with added dose of palmitate,and the cell count was reduced with prolonged treatment of palmitic acid.Hepatocytes treated with palmitic acid presented with densely stained nuclei as compared with the control group. Conclusion :Palmitic acid can induce apoptosis of hepatocytes and decrease cell viability.Palmitate-induced autophagy of hepatocytes may be involved in Erk1/ 2 signaling pathway.
作者
杜卉
张扬
孟祥健
吕康甲
吴晓莉
王李卓
邢春燕
何春玲
高家林
DU Hui;ZHANG Yang;MENG Xiangjian;LYU Kangjia;WU Xiaoli;WANG Lizhuo;XING Chunyan;HE Chunling;GAO Jialin(Department of Endocrinology,The First Affiliated Hospital of Wannan Medical College,Wuhu 241001,China)
出处
《皖南医学院学报》
CAS
2019年第1期5-8,共4页
Journal of Wannan Medical College
基金
国家自然科学基金项目(81800766)
安徽省自然科学基金项目(1708085MH188)
皖南医学院重点科研项目培育基金(WK2016ZF01)
皖南医学院中青年科研基金项目(WK2016F01)
作者简介
杜 卉(1992-),女,2015级硕士研究生,(电话)18895372049,(电子信箱)duhui_sd@163.com;通信作者:高家林,男,主任医师,教授,硕士生导师,(电子信箱)jialing.gao@yahoo.com。
