摘要
目的 FOXQ1基因在甲状腺乳头状癌(PTC)组织中呈现高表达,有关FOXQ1在PTC的生物学功能尚不清楚。文中旨在探讨FOXQ1-siRAN对PTC的TPC-1细胞增殖的影响及其可能的机制。方法通过lipofectamineTM2000将3条人工合成的FOXQ1-siRNA和NC-siRNA分别转入到PTC的TPC-1细胞,实验分为空白对照组(加入等量的磷酸盐缓冲液PBS)、NC-siRAN组(转染无意义序列siRNA)、FOXQ1-1组(空白培养基+lipofectamineTM2000+FOXQ1-1)、FOXQ1-2组(空白培养基+lipofectamineTM2000+FOXQ1-2)、FOXQ1-3组(空白培养基+lipofectamineTM2000+FOXQ1-3)。利用qRT-PCR和Western blot法分别检测FOXQ1 mRNA和蛋白的表达水平,MTT法观察细胞增殖活性的变化,qRT-PCR和Western blot分别检测细胞中细胞周期蛋白(cyclin D1)和c-Myc的表达水平。结果与空白对照组、NC-siRNA组FOXQ1 mRNA相对表达量比较,FOXQ1-3组明显抑制FOXQ1mRNA的表达(P<0.05)。FOXQ1-1组、FOXQ1-2组、FOXQ-3组的FOXQ1蛋白相对表达量(0.54±0.07、0.40±0.07、0.26±0.06)较NC-siRNA组(0.78±0.08)明显降低,FOXQ1-3组下降最为明显(P<0.05)。MTT结果显示,与空白对照组、NC-siRAN组比较,FOXQ1-3组可明显抑制TPC-1细胞的增殖活性(P<0.05)。FOXQ1-3组c-Myc、cyclin D1蛋白表达量(0.57±0.04、0.51±0.10)较空白对照组(1.05±0.07、0.94±0.12)、NC-siRNA组(0.92±0.06、0.91±0.11)明显降低(P<0.05)。结论沉默FOXQ1基因可以有效地抑制PTC的TPC-1细胞的增殖活性,这一作用可能通过抑制cyclin D1和c-Myc的表达水平来实现的。
Objective Forkhead box Q1(FOXQ1)is highly expressed but its biological role remains unclear in papillary thyroid carcinoma(PTC).This article aims to investigate the effect of FOXQ1-siRAN on the proliferation of TPC-1 cells and its possible mechanism.Methods Synthetic FOXQ1-siRNA and NC-siRNA were transfected into TPC-1 cells by lipofectamine^TM 2000.The cells were divided into five groups:NC-siRAN,FOXQ1-1,FOXQ1-2,FOXQ1-3 and blank control.After transfection,the expressions of the FOXQ1 mRNA and protein,as well as those of c-Myc and cyclinD1 proteins,were determined by qRT-PCR and Western blot,and the changes in the proliferation of the TPC-1 cells observed by MTT.Results Compared with the blank control and NC-siRAN groups,the FOXQ1-3 group showed a significantly inhibited expression of FOXQ1 mRNA(P<0.05).The protein expression of FOXQ1 was markedly decreased in the FOXQ1-1,FOXQ1-2 and FOXQ1-3 groups in comparison with that in the NC-siRAN group(0.54±0.07,0.40±0.07 and 0.26±0.06 vs 0.78±0.08,P<0.05).The proliferation of the TPC-1 cells was remarkably lower in the FOXQ1-3 than in the blank control and NC-siRNA groups(P<0.05),and so were the relative protein expressions of c-Myc(0.57±0.04 vs 1.05±0.07 and 0.92±0.06,P<0.05)and cyclinD1(0.51±0.10 vs 0.94±0.12 and 0.91±0.11,P<0.05).Conclusion Silencing the FOXQ1 gene can effectively inhibit the proliferation of TPC-1 cells in TPC,probably by suppressing the expressions of c-Myc and cyclinD1.
作者
钟烨
刘春辉
苏军涛
刘洋
田炜
王晓涛
张国志
ZHONG Ye;LIU Chun-hui;SU Jun-tao;LIU Yang;TIAN Wei;WANG Xiao-tao;ZHANG Guo-zhi(Department of General Surgery,Affiliated Hospital of North China University of Sciences and Technology,Tangshan 063000,Hebei,China;North China University of Science and Technology Medical Research Center,Tangshan 063000,Hebei,China)
出处
《医学研究生学报》
CAS
北大核心
2019年第1期64-68,共5页
Journal of Medical Postgraduates
基金
河北省科技成果推广课题(20150523)
华北理工大学研究生创新项目(2018S35)
作者简介
钟烨,医学硕士;通信作者:张国志,E-mail:279054524@qq.com.
