摘要
目的研究srGAP2(Slit-Robo GTPase activating Protein 2)、γ-氨基丁酸以及单核细胞趋化蛋白-1(MCP-1)在难治性癫痫患儿脑组织中的表达情况及意义。方法选取11例患儿的颞叶脑组织和同期11例正常标本的颞叶脑组织,使用免疫组化和免疫印迹等方法检测srGAP2、γ-氨基丁酸以及MCP-1的表达情况。结果难治性癫痫患儿脑组织中,srGAP2的阳性表达率显著高于正常脑组织。难治性癫痫患儿脑组织和正常脑组织可以看到染成棕褐色的神经元,但难治性癫痫患儿脑组织γ-氨基丁酸能神经元表达量明显低于正常脑组织。正常脑组织中MCP-1的表达较弱,而难治性癫痫患儿脑组织中MCP-1的表达较强。结论难治性癫痫患儿脑组织srGAP2和MCP-1表达上升而γ-氨基丁酸表达下降,srGAP2、γ-氨基丁酸及MCP-1与难治性癫痫发病密切相关,可能作为潜在治疗靶标。
Objective To investigate the expression and significance of srGAP2,GABA and MCP-1 in the brain of child patients with intractable epilepsy.Methods 11 cases of patients with temporal lobe brain tissue were randomLy selected,11 cases of normal temporal lobe brain tissue were enrolled during the same period.The expressions of srGAP2,GABA as well as MCP-1 were detected by immunohistochemistry and Western blot.Results The positive expression rate of srGAP2 in brain tissue of patients with refractory epilepsy was significantly higher than that in normal brain tissue.Brown neuron cells can be seen in the cytoplasm of neuronal cell in the brain tissue of patients with intractable epilepsy as well as normal brain tissue.However,the expression level of GABA neurons in brain tissue of patients with intractable epilepsy is lower than that in normal brain tissue.In normal brain tissues,the expression of MCP-1 is weak,and the expression of MCP-1 is stronger in the brain tissue of the patients with intractable epilepsy.The expression of MCP-1 is higher in the brain tissue of the patients with intractable epilepsy than that in the normal brain tissues.Conclusion The expressions of srGAP2 and MCP-1 in the brain tissue of the children with intractable epilepsy are increased,while the expression of GABA is decreased,srGAP2,GABA,and MCP-1 are closely related to intractable epilepsy and may serve as potential therapeutic targets.
作者
刘伟
吕秀丽
李战华
LIU Wei;LYU Xiuli;LI Zhanhua(Department of Pediatrics,the First People's Hospital of Zhumadian,Zhumadian 463000,China;ICU of Zhumadian Central Hospital,Zhumadian 463000,China)
出处
《中国实用神经疾病杂志》
2018年第23期2605-2611,共7页
Chinese Journal of Practical Nervous Diseases
基金
河南省高等学校重点科研项目(编号:16A310009)
作者简介
刘伟,Email:1749281713@qq.com;通信作者:李战华,Email:1282392875@qq.com
