摘要
以鸡肉腐败菌荧光假单胞菌和腐生葡萄球菌作为指示菌,研究绿原酸的抑菌机理,为绿原酸的开发利用提供理论依据。结果发现,绿原酸对2种指示菌的最小抑菌质量浓度、最小杀菌质量浓度为5 mg/ml;根据扫描电镜观察发现经过绿原酸处理后,2种指示菌表面有明显凹陷,细胞受损严重;通过对胞外ATP、蛋白质质量浓度的检测发现经过绿原酸处理后2种指示菌ATP、蛋白质发生明显泄漏;膜电位以及激光扫描共聚焦显微镜的结果也表明绿原酸使2种指示菌细胞膜受损,膜电位被破坏。由此看出,绿原酸是通过作用于细胞膜,使细胞膜受损严重,从而导致膜电位被破坏,胞内大分子物质泄漏从而达到抑菌作用。
Using chicken spoilage bacteria(Pseudomonas fluorescens and Staphylococcus saprophyticus)as indicator bacteria,the antimicrobial mechanism of chlorogenic acid was studied to provide a theoretical basis for the development and utilization of chlorogenic acid.The results showed that the minimal inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)of chlorogenic acid against two indicator bacteria were 5 mg/ml.According to the SEM,the surface of the two indicator bacteria treated with chlorogenic acid showed obvious depression and the cells were severely damaged.Moreover,chlorogenic acid treatment caused the significant leakage of ATP and protein.The results of membrane potential and LSCM showed that chlorogenic acid treatment caused the membrane damage of the two indicators and the membrane potential was also destroyed.Thus,chlorogenic acid resulted in cell death by destroying the cell membrane,which lead to the destruction of membrane potential and leakage of intracellular macromolecules.
作者
苏萌萌
孙芝兰
刘芳
吴海虹
张新笑
诸永志
王道营
徐为民
罗章
SU Meng-meng;SUN Zhi-lan;LIU Fang;WU Hai-hong;ZHANG Xin-xiao;ZHU Yong-zhi;WANG Dao-ying;XU Wei-min;LUO Zhang(College of Food Science,Agricultural and Animal Husbandry College of Tibet University,Linzhi 860000,China;Institute of Agricultural Products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing 210095,China)
出处
《江苏农业学报》
CSCD
北大核心
2018年第6期1386-1391,共6页
Jiangsu Journal of Agricultural Sciences
基金
江苏省重点研发计划项目(BE2017392)
江苏省农业科技自主创新基金项目[CX(17)3015]
国家现代农业(肉鸡)产业技术体系建设专项(CARS-41)
江苏省现代农业研究开发示范类项目(BE2016320)
关键词
绿原酸
荧光假单胞菌
腐生葡萄球菌
抑菌机理
chlorogenic acid
Pseudomonas fluorescens
Staphylococcus saprophyticus
antimicrobial mechanism
作者简介
苏萌萌(1995-),女,吉林公主岭人,硕士研究生,研究方向为植物提取物对肉品种主要腐败菌的抑菌机理。(Tel)17551041209;(E-mail)275094940@qq.com;通讯作者:徐为民,(Tel)13851577250;(E-mail)weiminxu2002@aliyun.com;通讯作者:罗章,(Tel)13989041759;(E-mail)luozhang1759@sohu.com
