摘要
针对延安产的青蒿(Artemisia carvifolia)和白蒿(Herba artimisiae sieversianae)2种植物,采取水蒸气蒸馏法提取其挥发油,采用菌丝生长速率法及96孔板法检测挥发油的抑菌活性,结果表明:当浓度是2.0 mg/mL时,青蒿挥发油对9种被测植物病原真菌的抑制作用均较差,对油菜菌核(Sclerotinia sclerotioru)活性最好,也仅为57.30%;白蒿挥发油对受试真菌的抑制作用较强,对人参锈腐菌(Cylindrocarpon destructans)、梨黑星病(Venturia nashicola)、烟草赤星病菌(Alternaria alternata)的抑制率均达到90%以上;2种精油除了对大肠杆菌(Escherichia coli)表现出较弱的抑制活性外,对于其他受试菌均无活性,其最小抑菌浓度(MIC)均为500 mg/mL。采用气相色谱-质谱联用仪(GC-MS)分析了2种蒿挥发油的主要化学成分,结果从青蒿中鉴定出26种化合物,其中含量最多的为石竹烯氧化物(25.75%);从白蒿中鉴定出49种化合物,萘嵌戊烷含量最高(30.37%),分析结果显示延安产2种蒿挥发油的主要成分和含量与其他产地的差异较大。白蒿挥发油具有显著的抑菌活性,具备开发为绿色杀菌剂的潜力。
Volatile oils were extracted from two Artemisia species of Yan’an by steam distillation.Antifungal and antibacteria activities were measured by mycelium growth rate and96well plate method.The results showed that at the concentration of2.0mg/mL,the antifungal activity of Artemisia stelleriana oil was weak and the highest inhibition rate was only57.3%against Sclerotinia sclerotioru.Volatile oil of Herba artimisiae sieversianae showed significant antifungal activity against9tested fungus and inhibition rates reached above90%against Cylindrocarpon destructans,Venturia nashicola,Alternaria alternata.Antibacterial activities of the oils were not observed except for Escherichia coli and inhibition rates were500mg/mL.Chemical components of volatile oils were analyzed and determined by gas chromatography-mass spectrum.26compounds were indentified from A.carvifolia oil.The most content was caryophyllene oxide and the content reached25.75%.49compounds were indentified from H.artimisiae sieversianae and the highest contest of penylene pentane was30.37%.The result showed volatile oil of H.artimisiae sieversianae had potential to be developed as new type green fungicide agents.
作者
艾飞翔
周靖怡
马秋
张恩生
陈小莉
胡华林
李䶮
杨华
AI Fei-xiang;ZHOU Jin-yi;MA Qiu;ZHANG Eng-sheng;CHENG Xiao-li;HU Hua-lin;LI Ye;YANG Hua(School of Chemistry and Chemical Engineering, Yan’an University, Yan’an 716000, China;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Key Laboratory of Chemistry for Natural Products of Guizhou Medical University, Guizhou 510014, China))
出处
《广东农业科学》
CAS
2018年第11期104-109,共6页
Guangdong Agricultural Sciences
基金
陕西省自然科学基金(2018JM2055)
延安大学大学生创新训练计划项目(D201607)
延安大学校级引导项目(YDY2017-08)
贵州省科学技术基金(黔科合J字[2015]2103号)
关键词
白蒿精油
青蒿精油
抑菌活性
气相色谱-质谱联用法(GC-MS)
Artemisia carvifolia
Herba artimisiae sieversianae
volatile oils
antifungal activity
gaschromatography-mass spectrum (GC-MS)
作者简介
艾飞翔(1997-),男,在读本科生,E-mail:3173615808@qq.com;通讯作者:杨华(1979-),女,硕士,副教授,E-mail:yanghua_08@163.com.
