摘要
目的探讨多发性骨髓瘤(MM)患者骨髓组织中微小RNA-181a(miR-181a)和微小RNA-373(miR-373)的表达及临床意义。方法收集2013年1月至2018年3月收治的MM患者64例(MM组),并选取同时期进行骨髓穿刺并最后明确骨髓功能无异常的健康者64例(正常对照组)。采用实时荧光定量PCR(QPCR)检测两组骨髓组织中miR-181a、miR-373的表达水平,并分析两者与MM临床病理特征的关系;采用Pearson法分析miR-181a和miR-373表达的相关性。结果 MM组骨髓组织中miR-181a的表达水平为112. 68±16. 53,高于正常对照组的75. 94±10. 02,差异有统计学意义(P<0. 01); miR-373的表达水平为0. 36±0. 06,低于正常对照组的1. 12±0. 10,差异有统计学意义(P<0. 01)。MM患者骨髓组织中miR-181a和miR-373的表达呈负相关(r=-0. 928,P<0. 001)。miR-181a和miR-373表达水平与MM患者的年龄、临床分期、分化程度及淋巴结转移有关(P<0. 001),与性别及一般分型无关(P>0. 05)。结论 miR-181a、miR-373在MM骨髓组织中分别为高表达和低表达,两者表达存在负相关,并与临床分期、淋巴结转移和分化程度有关,可作为MM潜在的分子筛查标志物。
Objective To investigate the expression and clinical significance of microRNA-181a(miR-181a)and microRNA-373(miR-373)in bone marrow of patients with multiple myeloma(MM).Methods Sixty-four MM patients(MM group)from January2013to March2018were selected.Sixty-four healthy subjects(normal control group)were selected who underwent bone marrow biopsy at the same time and were finally identified as normality in bone marrow function.The expression levels of miR-181a and miR-373in the two groups of bone marrow tissues were detected by real-time fluorescence quantitative PCR(QPCR).The relationship between expression of miR-181a and miR-373and clinicopathological parameters of MM was analyzed.Pearson correlation analysis was used to analyze the correlation between miR-181a and miR-373expression.Results The expression of miR-181a in bone marrow tissues of MM group was112.68±16.53,higher than75.94±10.02of normal control group(P<0.01).The expression of miR-373in bone marrow tissue of MM group was0.36±0.06,lower than1.12±0.10of normal control group(P<0.01).There was a negative correlation between the expression of miR-181a and miR-373in bone marrow tissue of MM patients(r=-0.928,P<0.001).The expression levels of miR-181a and miR-373were correlated with age,clinical stage,differentiation and lymph node metastasis(P<0.001),but not with gender and general type(P>0.05).Conclusion MiR-181a is highly expressed in MM bone marrow tissues,while miR-373is abnormally low.There is a negative correlation between their expression and they are correlated with clinical stage,lymph node metastasis and differentiation,which can be used as potential molecular screening markers for MM.
作者
刘仁涛
袁慕知
方宝枝
周一飞
余晓
LIU Rentao;YUAN Muzhi;FANG Baozhi;ZHOU Yifei;YU Xiao(Department of Hematology and Oncology, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215002, China)
出处
《临床肿瘤学杂志》
CAS
北大核心
2018年第11期1000-1004,共5页
Chinese Clinical Oncology
