摘要
目的探讨NF-κB和IκB-a蛋白表达水平变化与老年痴呆发病的相关性,讨论其在临床上应用的意义。方法选取我院确诊的老年痴呆患者120例(AD组)、体检正常的健康对象50例(对照组),收集时间2013年4月-2017年3月,蛋白质印迹法检测两组对象脑脊液中的NF-κB和IκB-a蛋白表达情况并分析其与临床病理学特征的关系。结果老年痴呆患者脑脊液中NF-κB和IκB-a蛋白表达明显高于对照组的表达,差异具有统计学意义(P<0.05);NF-κB和IκB-a蛋白表达与患者年龄、性别、和病情恶化阶段有关,差异具有统计学意义(P<0.05)。结论 NF-κB和IκB-a蛋白表达与老年痴呆发病具有密切的联系,有望成为检测老年痴呆的指标。
Objective To analyse the correlation between the changes of the expression level of NF-kappa B and I kappa B-a protein and the pathogenesis of Alzheimer's disease.Methods A total of 120 patients with Alzheimer's disease diagnosed in our hospital and 50 healthy subjects with normal physical examination were selected from April 2013 to March 2017.The expressions of NF-κB and IκB-a proteins in cerebrospinal fluid(CSF)of the two groups were detected by Western blot and the relationship between NF-κB and IκB-a protein expressions and clinicopathological characteristics was analyzed.Results The expressions of NF-kappa B and I kappa B-a protein in cerebrospinal fluid of patients with Alzheimer's disease were significantly higher than those of control group,and the statistical difference was significant(P<0.05).The expressions of NF-kappa B and I kappa B-a were related to the age,sex,and deterioration stage of the patient,and the statistical difference was significant(P<0.05).Conclusion The expressions of NF-kappa B and I kappa B-a protein is closely related to the pathogenesis of Alzheimer's disease,and are expected to be indicators for the detection of Alzheimer's disease.
作者
朱登玲
姜阿利
王慧峰
刘潘
徐凤琴
王霞
ZHU Dengling;JIANG Ali;WANG Huifeng;LIU Pan;XU Fengqin;WANG Xia(Kaifeng MEBO Hospital of internal medicine,Kaifeng,Henan,475002)
出处
《实验与检验医学》
CAS
2018年第6期846-849,共4页
Experimental and Laboratory Medicine
基金
河南省医学科技攻关计划项目
编号201504078
作者简介
朱登玲,女,1966年生,本科,副主任医师,研究方向:内科。
