摘要
为研究芒果胶孢炭疽菌在侵染过程中致病相关基因的差异表达。采用实时荧光定量PCR的方法,分析病菌侵染芒果叶片和果实过程中PL、PG等11个基因的表达量变化。研究发现,病原菌侵染叶片时,PG和PL基因均持续高效表达,SIN3P基因表达较低,其余基因在侵染6 h时表达量较高,随后下降;病原菌侵染果实时,PL、PG、SDH、ECH等基因高效表达,其余基因则有升有降。说明致病基因在胶孢炭疽菌侵染芒果不同组织时表达有差异,在侵染的不同时段也不同程度地发挥功能。
This work aims to study the differential expressions of pathogenicity-related genes during the process of infecting mango by Colletotrichum gloeosporioides.Quantitative real-time PCR was utilized to analyze the expression variations of 11 genes including PL and PG during pathogen infecting mango leaves and fruits.The results showed that the PG and PL genes were highly expressed continuously and the expression of SIN3P gene was low,while the other genes expressed high at 6 h after infection and then decreased during the process of C.gloeosporioides infection to mango leaves.PL,PG,SDH,ECH and other genes highly expressed when the pathogen infected the fruit,while the rest of the genes either increased or decreased.These results indicate that the pathogenic genes express differentially while C.gloeosporioides infects different tissues,and play different roles in the different infection period at varied level.
作者
苏初连
康浩
梅志栋
杨石有
刘晓妹
蒲金基
张贺
SU Chu-lian;KANG Hao;MEI Zhi-dong;YANG Shi-you;LIU Xiao-mei;PU Jin-ji;ZHANG He(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture,Haikou 571101;Chengmai County Agriculture Bureau,Chengmai 571900)
出处
《生物技术通报》
CAS
CSCD
北大核心
2018年第10期182-186,共5页
Biotechnology Bulletin
基金
国家自然科学基金项目(31460455)
中国热带农业科学院基本科研业务费专项资金(1630042017019
1630042017030)
海南省重大科技计划项目(ZDKJ2017003)
国家重点研发计划(2017YFD0202107-3)
关键词
胶孢炭疽菌
致病基因
表达分析
Colletotrichum gloeosporioides
pathogenic genes
expression analysis
作者简介
苏初连,女,硕士研究生,研究方向:微生物学;E-mail:2529025381@qq.com;康浩,同为本文第一作者;通讯作者:张贺,男,硕士,助理研究员,研究方向:热带果树病理学;E-mail:3086308343@qq.com。
