摘要
利用30对荧光标记微卫星引物对天津大港、辽宁盘锦、山东烟台、江苏射阳近海4个自然群体的120个海蜇样本进行遗传多样性分析。结果显示,各群体平均等位基因数为4.0333~6.8333个,平均观测杂合度为0.3906~0.5059,平均期望杂合度为0.4353~0.5986,4个群体的多态信息含量为0.3902~0.5497。遗传距离分析显示,盘锦群体和烟台群体的遗传距离最小(0.0653),亲缘关系最近;而盘锦群体与射阳群体之间的遗传距离最大(0.1629),亲缘关系最远。基于Nei′s遗传距离构建的系统进化树显示,盘锦和烟台群体聚为一支,之后再与大港群体聚为一支,最后与射阳群体聚为一类。综上,4个群体的遗传多样性水平处于中高程度,遗传距离与试验群体地理距离基本相符。本研究的结果将为海蜇分子遗传育种和种质保护提供参考依据。
The genetic diversities of four wild populations in jellyfish(Rhopilema esculenta)sampled from coasts of Dagang in Tianjin,Panjin in Liaoning,Sheyang in Jiangshu,and Yantai in Shandong were analyzed in 120 individuals using thirty fluorescently labeled microsatellite DNA primers to assess the genetic diversities of the four wild populations of jellyfish.The results showed that the average number of alleles was ranged from 4.0333 to 6.8333 in the four populations,with mean observed heterozygosity of 0.3906-0.5059,expected heterozygosity of 0.4353-0.5986,and average polymorphism information content of 0.3902-0.5497.Nei′s genetic distance indicated that Panjin and Yantai populations(0.1629)had the nearest genetic distance(0.0653)while the farthest genetic distance were shared by Panjin and Sheyang populations.Phylogenetic tree revealed that Panjin and Yantai populations were clustered together into as one group,then to Dagang population,finally to Sheyang population.In summary,the genetic diversity among the four populations is mid-high and the genetic distances are in accordance with the geographical distances among the experimental populations.The findings provide reference for marker-assisted genetic breeding and germplasm conservation in the jellyfish.
作者
狄明玉
周遵春
李云峰
田梅琳
侯红漫
李玉龙
鲍相渤
赫崇波
DI Mingyu;ZHOU Zunchun;LI Yunfeng;TIAN Meilin;HOU Hongman;LI Yulong;BAO Xiangbo;HE Chongbo(College of Food Science,Dalian Polytechnic University,Dalian 116034,China;Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China)
出处
《水产科学》
CAS
CSCD
北大核心
2018年第6期762-768,共7页
Fisheries Science
基金
国家自然青年科学基金资助项目(31302173)
辽宁省海洋与渔业厅项目(201604)
关键词
海蜇
自然群体
微卫星
遗传多样性
Rhopilema esculenta
wild population
microsatellite
genetic diversity
作者简介
狄明玉(1993—),女,硕士研究生;研究方向:食品生物技术.E-mail:1273473651@qq.com.;通讯作者:李云峰(1979-),男,副研究员;研究方向:水产动物遗传育种.E-mail:yunfengli@126.com.
