摘要
为了研究创建薄皮甜瓜‘花雷’的再生体系,以薄皮甜瓜‘花雷’为试材,子叶、胚轴、真叶为外植体,观察其在含有不同种类和浓度生长调节剂的培养基中的再生频率。诱导愈伤组织的最佳外植体为子叶,最佳诱导培养基为MS+0.5 mg·L^(-1)6-BA+0.5 mg·L^(-1)IAA,最佳分化培养基为MS+0.5 mg·L^(-1)6-BA+0.2 mg·L^(-1)IBA,最佳生根培养基为MS+0.2 mg·L^(-1)IBA。初步选出一套完整的适宜薄皮甜瓜‘花雷’再生体系建立的培养基。
The regeneration system of‘Hualei’of melon was established.The regeneration frequency in culture medium containing different kinds and concentration of growth regulator was observed by using‘Hualei’of melon as the experimental material and cotyledon,hypocotyl and true leaf as explants.The best explants for callus induction were cotyledon,the best induction medium was MS+0.5mg·L^-16-BA+0.5mg·L^-1IAA,the best differentiation medium was MS+0.5 mg·L-1 6-BA+0.2mg·L-1 IBA,and the best rooting medium was MS+0.2 mg·L-1 IBA.A complete culture medium suitable for the regeneration system of the melon‘Hualei’was selected.
作者
武云鹏
张若纬
彭冬秀
张秀婷
WU Yunpeng;ZHANG Ruowei;PENG Dongxiu;ZHANG Xiuting(Tianjin Enterprise Key Laboratory of Vegetable Genetics&Breeding,State Key Laboratory of Vegetable Gemplasm Innovation,Tianjin Kernel Agricultural Science&Technology Corporation Limited Vegetable Research Institute,Tianjin 300381,China;Tianjin Vocational College of Bioengineering,Tianjin 300462,China)
出处
《中国瓜菜》
CAS
北大核心
2018年第11期36-39,共4页
China Cucurbits And Vegetables
基金
天津市农业科学院院长基金项目(16012)
关键词
薄皮甜瓜
子叶
再生频率
愈伤组织
外植体
Oriental melon
Cotyledons
Regeneration frequency
Callus
Explant
作者简介
武云鹏,男,助理研究员,研究方向为甜瓜遗传育种。E-mail:wuyunpeng7595@126.com。
