摘要
为研究投喂频率和糖源组成对凡纳滨对虾Litopenaeus vannamei糖代谢的影响,以初始体质量为(0. 38±0. 01) g的凡纳滨对虾为对象,采用3×2双因子试验方法,其投喂频率分别为2、5、7次/d,糖源饲料分别为蔗糖和玉米淀粉,经过56 d的生长试验,研究了不同投喂频率和两种糖源饲料对凡纳滨对虾生长、代谢酶、消化酶活性的影响。结果表明:蔗糖组和玉米淀粉组中投喂7、5次/d时对虾末体质量、特定生长率均显著高于投喂2次/d (P<0. 05);饲料系数以投喂2次/d时最高,且蔗糖组投喂2、5次/d时对虾的饲料系数均显著高于玉米淀粉组(P<0. 05);投喂频率和糖源交互作用对对虾肝胰腺己糖激酶活性影响显著(P<0. 05),凡纳滨对虾肝胰腺中苹果酸脱氢酶和胃蛋白酶活性随投喂频率的增加显著升高(P<0. 05),各投喂频率下磷酸果糖激酶、脂肪酶活性差异显著(P <0. 05),趋势为投喂5次/d> 7次/d> 2次/d。研究表明,提高投喂频率可以获得较高的特定生长率和代谢酶活性,但两组糖源间无显著性差异。
An 8-week 3×2 two-factorial feeding trial was conducted to study the effects of feeding frequency(7 times/d,5 times/d,and twice/d)and carbohydrate(sucrose and cornstarch)on growth,and activities of metabolic enzymes and digestive enzymes in Pacific white leg shrimp Litopenaeus vannamei with initial body weight of(0.38+0.01)g.The results showed that the shrimp fed diets containing sucrose and corn starch had significantly higher final body weight and specific growth rate at feeding frequency of 7 times/d and 5 times/d than those at twice a day(P<0.05).The maximal food conversion ratio was observed in the shrimp fed twice a day,with significantly higher twice a day and 5 times/d in sucrose group than that in cornstarch group(P<0.05).The activities of malate dehydrogenase and pepsin were shown to be significantly elevated with increase in feeding frequency in the hepatopancreas of the shrimp(P<0.05),the maximal activities of kinase and lipase and significant difference(P<0.05).The interaction between feeding frequency and sugar sources showed a significant effect on the activity of glucokinase in the hepatopancreas of the shrimp with the descending order as 5 times/d>7 times/d>twice a day(P<0.05).The findings indicate that there is no significant difference in sugar sources between the two groups,and that high feeding frequency leads to high specific growth rate,and metabolic enzyme and digestive enzyme activities.
作者
杨品贤
贾高旺
夏辉
齐国山
李雪鹤
路晶晶
张宇
郭冉
YANG Pin-xian;JIA Gao-wang;XIA Hui;QI Guo-shan;LI Xue-he;LU Jing-jing;ZHANG Yü;GUO Ran(Ocean College,Hebei Agricultural University,Qinhuangdao 066003,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第5期583-588,共6页
Journal of Dalian Ocean University
基金
河北省高等学校科学技术研究项目(QN2017335)
河北农业大学人才振兴学科计划项目(1081005)
关键词
凡纳滨对虾
投喂频率
生长性能
糖代谢
糖源饲料
Litopenaeus vannamei
feeding frequency
growth performance
carbohydrate metabolism
carbohydrate feed
作者简介
杨品贤(1994—),女,硕士研究生。E-mail:237631217@qq.com;通信作者:郭冉(1980—),女,副教授。E-mail:toguoran@163.com。
