摘要
通过薄膜分散法制备二甲基姜黄素(ASC-J9)脂质体,其粒径分布均匀且在水中的分散效果好,平均粒径为145. 7 nm,分散系数为0. 361。将ASC-J9脂质体作为水溶性的荧光探针,通过荧光猝灭法可选择性识别Fe^(3+)、Fe^(2+)及Cu^(2+)。经过条件筛选得到最佳荧光测试条件为:ASC-J9脂质体浓度为5. 0×10^(-5)mol/L,平衡时间5 min,测试温度25℃。由Job's曲线和荧光滴定结果判断该探针与Fe^(3+)和Cu^(2+)的络合比均为1∶1,与Fe~^(2+)的络合比为2∶1; Stern-Volmer方程判断该荧光猝灭类型为静态猝灭,结合常数分别为KFe(Ⅲ)=9. 63×10~4L/mol,KFe(Ⅱ)=3. 65×10~5L/mol,KCu(Ⅱ)=2. 32×10~5L/mol;该识别体系检测快速、灵敏度高,对Fe^(3+)、Fe^(2+)、Cu^(2+)的线性范围分别为:5. 0×10^(-7)~3. 0×10^(-5)、5. 0×10^(-7)~1. 75×10^(-5)、5. 0×10^(-7)~2. 5×10^(-5)mol/L,检出限分别为6. 41×10^(-7)、3. 28×10^(-7)、5. 08×10^(-7)mol/L。
A water-soluble molecular fluorescence probe,dimethyl curcumin(ASC-J9)liposome was developed for the recognition of Fe 3+,Fe 2+and Cu 2+through fluorescence quenching.The ASC-J9 liposome was prepared by thin-film dispersion,which had a uniform particle size distribution with a particle size of 145.7 nm and a polymer dispersity index(PDI)of 0.361.The optimal fluorescence conditions obtained by conditional screening were as follows:ASC-J9 liposome concentration:5.0×10-5 mol/L,balance time:5 min,temperature:25℃.The binding modes of ASC-J9 liposome with Fe 3+,Cu 2+and Fe 2+were achieved by Job s plot and fluorescence titration.Results showed that the binding ratios for the probe to Fe 3+and Cu 2+were both 1∶1,while that for the probe to Fe 2+was 2∶1.The fluorescence quenching type was determined by Stern-Volmer plot as static quenching,and the association constants of K Fe(Ⅲ),K Fe(Ⅱ)and K Cu(Ⅱ)were 9.63×10 4 L/mol,3.65×10 5 L/mol and 2.32×10 5 L/mol,respectively.With the advantages of rapid detection and high sensitivity,this method was applied in the identification of Fe 3+,Fe 2+and Cu 2+ions in water samples.The calibration curves for Fe 3+,Fe 2+and Cu 2+were 5.0×10-7-3.0×10-5 mol/L,5.0×10-7-1.75×10-5 mol/L and 5.0×10-7-2.5×10-5 mol/L,with their limits of detection of 6.41×10-7 mol/L,3.28×10-7 mol/L and 5.08×10-7 mol/L,respectively.
作者
李正义
邵丹迪
殷乐
肖唐鑫
孙小强
LI Zheng-yi;SHAO Dan-di;YIN Yue;XIAO Tang-xin;SUN Xiao-qiang(Institute of Petrochemical Engineering,Changzhou University,Changzhou 213164,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2018年第9期1046-1050,共5页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21572026)
江苏省先进催化与绿色制造协同创新中心项目(ACGM2016-06-05)
作者简介
通讯作者:孙小强,博士,教授,研究方向:超分子化学,E-mail:sunxiaoqiang@yahoo.com
