摘要
探讨参与肌浆网中钙离子释放的兰诺定受体(ryanodine receptor,RyR)、受磷蛋白(phospholamban,PLN)的mRNA转录水平与失用性肌萎缩的关系。将依次编号的30只新西兰大白兔按随机数字法分为6组。分别设置为对照组和实验组5组。实验组采用长腿石膏固定法建立兔失用性肌肉萎缩模型,于2周、4周、6周、8周、10周分别处死兔子5只。对照组于第10周处死兔子5只。取固定侧肌肉组织,用定时定量PCR(real-time quantitative polymerase chain reaction,RT-PCR)法测定兔固定侧肌肉的RyR与PLN的基因转录水平。RyR的mRNA转录水平在第2周升高,自第2周开始转录水平逐渐下降。PLN的mRNA转录水平表现为逐渐下降的趋势。在失用性萎缩发展过程中,PLN和RyR mRNA随着肌肉萎缩程度的不断加深呈现趋势性变化,可望用于失用性肌萎缩程度的推断。
The transcription level of ryanodine receptor(RyR)mRNA and phospholamban(PLN)mRNA of disused muscles in rabbits and to explore the relationship with the fixation time were investigated.Thirty New Zealand rabbits were randomly divided into 6 groups(5 rabbits in each group),including five test groups(2,4,6,8,10 weeks)and one control group.The disease model was established by fixing the right rear knee joint in extension position using plaster cast.The rabbits were respectively sacrificed at 2,4,6,8,10 weeks after operation.The five rabbits of control group were sacrificed at the 10th week.The transcription level of RyR and PLN mRNA in the fixed muscles of rabbits was determined by real-time quantitative polymerase chain reaction(RT-PCR).The transcription level of RyR mRNA increased within 2 weeks and then decreased gradually in the following 2 weeks.The transcription level of PLN mRNA decreased gradually.The transcription level of RyR、PLN mRNA shows a time-related change after fixation of the skeletal muscle.It is a potential promising method to evaluate the degree of muscular atrophy.
作者
王威
王月
张美香
殷文江
李周儒
董国凯
李珊珊
马静媛
蒯锦霞
包海军
孙晓明
蔡红星
WANG Wei;WANG Yue;ZHANG Mei-xiang;YIN Wen-jiang;LI Zhou-ru;DONG Guo-kai;LI Shan-shan;MA Jing-yuan;KUAI Jin-xia;BAO Hai-jun;SUN Xiao-ming;CAI Hong-xing(Xuzhou Medical University,Department of Forensic Medicine Xuzhou,Xuzhou 221004,China)
出处
《科学技术与工程》
北大核心
2018年第27期106-109,共4页
Science Technology and Engineering
基金
徐州医科大学优秀人才科研启动基金(A53591505,A53591504)、徐州市科技局重点课题(KC15SM047)、江苏省教育厅课题(16KJB340002)和江苏省高校自然指导项目(15KJD180003)资助.
作者简介
第一作者简介:王威(1993—),男,汉族,江苏徐州人,硕士研究生。E-mail:xzww1993@126.com;通信作者简介:孙晓明(1980—),男,汉族,山东烟台人,博士,讲师。E-mail:lupusstar@163.com。
