摘要
目的本研究旨在探讨动物鼻式气溶胶暴露装置能否正常运行并产生特定粒径大小的气溶胶颗粒,探究运行过程中装置气溶胶气密性、分布均匀性及消毒剂对系统的消毒效果。方法使用动物鼻式气溶胶暴露装置产生大肠杆菌(Escherichia coli,E.coli)气溶胶、鼠肝炎病毒(mouse hepatitis virus)MHV-JHM气溶胶,分别用75%乙醇、3%过氧化氢对系统进行消毒,分别采集暴露舱内、易泄露处及消毒后的E.coli、MHV-JHM。对采集的E.coli进行微生物培养;对采集的MHV-JHM提取RNA,一步法RT-PCR检测样本中病毒RNA。结果装置产生的E.coli气溶胶颗粒中值直径为(1.27±0.61)μm,暴露舱内采样液涂板有菌落生成,置于暴露舱不同端口的NC膜上菌落数一致,易泄露处的沉降平皿上无菌落生长,消毒后采样平皿上无菌落生长;暴露舱内采样液中的MHVJHM病毒RNA为阳性,易发生泄露处采样液中病毒RNA为阴性,消毒后采样液中病毒RNA为阴性。结论动物鼻式气溶胶暴露装置能正常运行产生气溶胶;在运行过程中,暴露舱各端口有气溶胶通过并分布均匀,气密性好;使用相应的消毒剂可对系统进行有效消毒,消毒效果完全。
Objective To investigate whether the animal nose-only inhalation exposure device can operate normally and produce aerosol particles of a specific size,to explore the air tightness and uniform distribution of the aerosol during operation,and to examine the disinfection effect of disinfectant on the system.Methods Escherichia coli(E.coli)aerosol and mouse hepatitis virus MHV-JHM aerosol were developed by the nose-only inhalation exposure device,and a solution of 75%ethanol and 3%hydrogen peroxide was run through the nebulizer to disinfect the system.E.coli and MHV-JHM were collected respectively in the exposure chamber,at sites where leaking easily occurred,including after disinfection.The sampled E.coli was cultured.In addition,RNA from the sampled MHV-JHM was extracted and one-step RT-PCR was then used to test the presence of viral RNA from the samples.Results The result showed that the mass median aerodynamic diameter of the E.coli aerosol was 1.27±0.61μm,that colonies grew after coating of a plate with sample liquid collected in the exposure chamber,that the numbers of cultured colonies by NC membrane sampling of different ports were similar,and that no colony growth was found on the sedimentation plate at sites where leaking readily occurred or on the sampling plates after disinfection.There was a positive result for MHV-JHM virus RNA of the sampling solution from the liquid impinger,while there were negative results for MHV-JHM virus RNA of the sampling solutions from the sites where leaking easily occurred and after disinfection.Conclusions The results show that the animal nose-only inhalation exposure device can operate normally and develop an aerosol that is passed through and distributed evenly in each port of the exposure chamber during operation;moreover,the device was shown to have good air tightness.The system can be effectively disinfected by using the corresponding disinfectant,and the disinfection effect is comprehensive.
作者
郝新彦
向志光
高虹
HAO Xinyan;XIANG Zhiguang;GAO Hong(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS);Comparative Medicine Center,Peking Union Medical College(PUMC),Beijing 100021,China)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第9期83-89,共7页
Chinese Journal of Comparative Medicine
基金
“十三五”国家科技重大专项(编号:2018ZX10734401-011)
国家重点研发计划(编号:2016YFD0501003)。
作者简介
郝新彦(1993— ),女,硕士研究生,研究方向:感染性疾病动物模型制作。E-mail:1905133927@qq.com;通信作者,高虹(1968— ),女,研究员,研究方向:药物临床前安全性评价。E-mail:gaoh@cnilas.org
