摘要
对羊肚菌成熟子实体的转录组进行高通量测序,并对得到的unigene进行基因本体论富集分析、真核生物直系同源聚类分析和代谢通路分析。经组装,共得到14 637条unigene,其中8 495个unigene得到了功能注释;利用基因本体论富集分析、真核生物直系同源功能分类将注释unigene划分分别为58个和25个功能类别,涉及物质合成与代谢、呼吸与能量代谢、信号传导等诸多生理生化过程。在此基础之上,通过对羊肚菌采后生理涉及的unigene进行深入分析,发现大量参与呼吸代谢、碳水化合物代谢、组织软化、氧化褐变等相关途径的关键基因。研究结果为羊肚菌采后品质变化机制的研究、贮藏保鲜技术的开发提供了一定的理论基础。
In the present work,high throughput de novo sequencing of the Morchella importuna transcriptome was carried out,and the annotated sequences were classified using gene ontology(GO),eukaryotic orthologous groups(KOG),and Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways.After assembly,a total of 14 637 unigenes were formed,8 495 of which were annotated.By functional classification using GO and KOG,the annotated unigenes were divides into 58 and 25 categories,respectively,involved in many physiological and biochemical processes including synthesis and metabolism of biomolecules,respiration and energy metabolism and signal transduction.Furthermore,a large number of key genes involved in energy metabolism,the synthesis and hydrolysis of nutrients,the softening of texture,oxidation of polyphenols and browning were identified through analysis of the unigenes related to postharvest physiology.The data obtained in this study can provide important information for the understanding of the molecular mechanism of postharvest quality changes in Morchella importuna and for the development of new preservation technologies.
作者
王金秋
叶鸿亮
梁道崴
刘达玉
耿放
李翔
WANG Jinqiu;YE Hongliang;LIANG Daowei;LIU Dayu;GENG Fang;LI Xiang(College of Pharmacy and Biological Engineering,Chengdu University,Chengdu 610106,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第18期81-87,共7页
Food Science
基金
国家自然科学基金青年科学基金项目(31701655)
四川省教育厅重点项目(17ZA0080)
四川省教育厅创新团队项目(16TD0036)
关键词
羊肚菌
转录组测序
功能注释
营养代谢
贮藏保鲜
Morchella
transcriptome sequencing
functional annotation
nutritional metabolism
storage and preservation
作者简介
王金秋(1986—),女,讲师,博士,研究方向为农产品加工与贮藏。E-mail:shiyuejq023@163.com;通信作者简介:李翔(1974—),女,教授,博士,研究方向为食品加工与安全。E-mail:lixiang@cdu.edu.cn。
