摘要
【目的】明确低氧对斑马鱼细胞存活和抗氧化酶系统的影响,为揭示鱼类细胞低氧适应机理打下基础。【方法】采用不同浓度低氧处理斑马鱼胚胎上皮细胞(ZF-4细胞),检测低氧对其存活率的影响,并分析低氧应激下ZF-4细胞中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)、一氧化氮合酶(NOS)活性及丙二醛(MDA)和一氧化氮(NO)含量的变化。【结果】1.0%O2处理可促进ZF-4细胞存活;SOD活性呈先降低后升高的变化趋势,在处理24 h时达最高值;CAT活性呈先降低后升高再降低的波动变化趋势,于处理8 h时达最低值;GSH活性在处理16 h时达最高值,随后开始下降;MDA含量与对照组无显著差异(P>0.05);NO含量和NOS活性在处理16 h时达最高值。经0.1%O2处理8 h后ZF-4细胞的存活率达最高值;SOD活性在处理24 h时显著高于对照组(P<0.05,下同);经0.1%O2处理后CAT活性极显著降低(P<0.01,下同),GSH活性则从处理8 h后极显著高于对照组;MDA含量显著或极显著降低;NO含量和NOS活性极显著降低。【结论】在极端缺氧环境下,斑马鱼细胞主要通过提高SOD和GSH等抗氧化酶活性以提高细胞的低氧适应能力,从而促进细胞存活。
【Objective】The study was to investigate the effects of hypoxic on cell survival and the antioxidative enzyme,and lay a foundation for the cell adaptive responses to hypoxia in fish.【Method】The epithelial cells of zebrafish(ZF-4 cells)were treated with different hypoxia concentrations,the cell survival were detected in the treatment.Then,the changes of activities of superoxide dismutase(SOD),catalase(CAT),glutathione reductase(GSH),nitric oxide synthase(NOS),and the contents of malondialdehyde(MDA),nitric oxide(NO)were analyzed.【Result】The study demonstrated that cell survival rate were elevated under 1%O2.The activity of SOD were decreased firstly and increased,and reached the highest value at the 24th hour.The CAT activity went down firstly,then went up,and down again reaching minimum at the 8th hour.The GSH levels gradually decreased,peak of activity was observed at the 16th hour;but the content of MDA was not significantly changed compared to control(P>0.05).The content of NO and the activity of NOS increased and reached the peak at the 16th hour.The ZF-4 cells had the highest survival rate under 0.1%oxygen after 8 hour.The activity of SOD wassignificantly higher than that in control at 24th hour(P<0.05,the same below),but the activity of CAT was extremely reduced(P<0.01,the same below).The activity of GSH was extremely higher than that in control since the 8th hour;the contents of MDA significantly or extremely reduced under 0.1%oxygen;the content of NOS and NO were decreased significantly.【Conclusion】Under acute hypoxia condition,zebra fish mainly promote cell survival by improving the activity of SOD,GSH and other antioxidases to improve the hypoxia adaptation of cells,so that cells can survive.
作者
武晓会
刘洋
狄治朝
赵文静
王丛丛
许强华
WU Xiao-hui;LIU Yang;DI Zhi-chao;ZHAO Wen-jing;WANG Cong-cong;XU Qiang-hua(College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Sustainable Exploitation of Ocean Fisheries Resources,Ministry of Education,Shanghai 201306,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2018年第8期1641-1647,共7页
Journal of Southern Agriculture
基金
国家自然科学基金青年基金项目(31702312)
大洋渔业资源可持续开发教育部重点实验室开放基金项目(A1-0203-00-2009-4)
关键词
斑马鱼
ZF-4细胞
低氧
氧化应激
抗氧化酶
存活能力
zebra fish
ZF-4 cells
hypoxia
oxidative stress
antioxidant enzymes
survival ability
作者简介
武晓会(1992-),研究方向为鱼类分子遗传学,E-mail:1229201030@qq.com;通讯作者:王丛丛(1984-),博士,主要从事水产动物环境适应分子机制与分子进化研究工作,E-mail:ccwang@shou.edu.cn;通讯作者:许强华(1974-),教授,主要从事水生动物低氧适应机制研究工作,E-mail:qhxu@shou.edu.cn。
