摘要
本研究对2016年3月~2017年3月采集于上海市猪场的20份病料进行了病原体检测,并对检测到的圆环病毒3型(Porcine circovirus type 3,PCV3)进行了全基因分析。检测结果显示:猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome,PRRSV)阳性病料1份,而猪圆环病毒2型(Porcine circovirus type 2,PCV2)、猪伪狂犬病毒(Pseudorabies virus,PRV)、猪瘟病毒(Classical swine fever virus,CSFV)、猪细小病毒(Porcine parvovirus,PPV)、猪乙脑病毒(Japanese encephalitis virus,JEV)均为阴性;PCV3阳性病料2份,其中1份为PCV3和PRRSV混合感染。对这2株PCV3进行全基因测序和Blast分析,并绘制PCV3全基因、囊膜蛋白基因的遗传进化树,结果显示:与这2株病毒基因同源性最高的毒株为CN/Hubei-618/2016,同源性为99%;在全基因进化树中,这2株毒株和中国来源的PCV3毒株以及美国毒株PCV3/USA/MO2015(Gen Bank登录号:KX778720.1)在同一群中;在囊膜蛋白基因的进化树中,这2株PCV3属于PCV3a。
Twenty-three samples collected from pig farms in Shanghai were tested using qPCR.One aborted fetal sample was positive for Porcine reproductive and respiratory syndrome(PRRSV)and other samples were negative for PRRSV,Porcine circovirus type 2(PCV2),Pseudorabies virus(PRV),Classical swine fever virus(CSFV),Porcine parvovirus(PPV)and Japanese encephalitis virus(JEV).In addition,these samples were detected for Porcine circovirus type 3(PCV3).Two samples from were positive for PCV3,one of which was co-infected with PRRSV.The whole genomes of two PCV3 isolates(KY865242 and KY865243)were phylogenetically analyzed.The result indicated,these two PCV3 isolates were clustered into the same group with other Chinese PCV3 isolates and USA isolate PCV3/USA/MO2015(KX778720.1).These two PCV3 strains belonged to the clade 3a based on their partial capsid gene sequences.The genomic sequences of these PCV3 isolates were 99%identical to CN/Hubei-618/2016 through Blast analysis.
作者
葛菲菲
李鑫
刘健
鞠厚斌
杨德全
王建
周锦萍
GE Fei-fei;LI Xin;LIU Jian;JU Hou-bin;YANG De-quan;WANG Jian;ZHOU Jin-ping(Shanghai Animal Disease Control Center,Shanghai 201103,China)
出处
《中国动物传染病学报》
CAS
北大核心
2018年第4期6-10,共5页
Chinese Journal of Animal Infectious Diseases
关键词
猪圆环病毒3型
混合感染
基因进化分析
Porcine circovirus type 3
coinfection
phylogenetical analysis
作者简介
葛菲菲,女,博士,主要从事分子生物学检测与病毒基因变异研究;通信作者:周锦萍,E-mail:shzjpvet@163.comn。
