摘要
【目的】探讨小叶榄仁幼苗对盐碱环境的适应机理,为盐碱土地改良及耐盐碱小叶榄仁苗木引种栽培提供科学依据。【方法】以不同浓度Na Cl胁迫2年生小叶榄仁盆栽实生幼苗,30 d后测定其株高增量、地径增量、叶面积、生物量(干重和鲜重)及可溶性糖、丙二醛(MDA)和脯氨酸含量等指标,分析盐胁迫对其生长和渗透调节物质含量等指标的影响。【结果】随盐胁迫浓度的升高,小叶榄仁幼苗的株高增量、地径增量、叶面积和生物量均明显低于对照(CK),在0.6%~1.0%盐胁迫下,小叶榄仁幼苗生长严重受阻;可溶性糖含量随盐浓度的升高呈递增趋势,在0.8%盐胁迫下达最大值后迅速下降,0.8%是可溶性糖含量累积的临界盐浓度;脯氨酸和MDA含量随盐浓度的升高总体上呈逐渐升高的变化趋势,在1.0%高盐胁迫下达最大值,且与CK差异显著(P<0.05,下同),0.8%盐胁迫下脯氨酸开始发挥抗盐作用。相关性分析结果表明,在盐胁迫下小叶榄仁幼苗叶片的可溶性糖含量与脯氨酸含量和干重呈显著正相关,脯氨酸含量与MDA含量呈显著负相关。【结论】盐胁迫会引起小叶榄仁幼苗株高、地径、叶面积和生物量等生长指标下降,0.8%是胁迫小叶榄仁幼苗渗透性调节物质含量发生变化的临界盐浓度;小叶榄仁幼苗能在0.2%~0.4%盐胁迫环境下良好生长,可作为低盐土壤环境改良树种引种栽培。
【Objective】The aim of the present study was to investigate the adaptation mechanism of Terminalia neotaliala Capuron seedlings to saline-alkali environment,and to provide scientific basis for the improvement of saline-alkali soil and the introduction of saline-alkali-resistant seedlings of T.neotaliala.【Method】Two-year-old seedlings were treated with different concentrations of NaCl by using pot experiment.After 30 d,height increase,ground diameter increase,leaf area,biomass(dry weight and fresh weight),soluble sugar content,malondialdehyde(MDA)content,and proline content were determined.The indexes were used to analyze the effects of salt stress on the growth and osmoregulatory substances in T.neotaliala.【Result】With the increase of salt stress concentration,the plant height increase,ground diameter increase,leaf area and biomass of T.neotaliala seedlings were significantly lower than control(CK).Under 0.6%-1.0%salt stress,the growth of T.neotaliala seedlings was seriously hindered.The soluble sugar content increased with the increase of salt concentration,and reached the highest at 0.8%salt stress,and then dropped sharply.The 0.8%salt concentration was the critical concentration for accumulation of soluble sugar content.The content of proline and MDA gradually increased with the increase of salt concentration.Both increased to the maximum at 1.0%high salt stress,and were significantly different compared to CK(P<0.05,the same below).Proline began to exert salt resistance when the salt concentration was 0.8%.The results of correlation analysis showed that there was a significant positive correlation between soluble sugars content and dry weight,between proline content and dry weight,however,there was significant negative correlation between proline content and MDA content.【Conclusion】Salt stress causes the decline of the growth indexes such as plant height,ground diameter,leaf area and biomass.The 0.8%salt concentration is the critical concentration for the change of osmoregulatory substances in T.neotaliala seedlings.The seedlings of T.neotaliala can grow well under the condition of 0.2%-0.4%salt stress,therefore,T.neotaliala can be introduced as a tree species cultured in low salt soil environment.
作者
黄相玲
林妃妃
张明月
朱栗琼
招礼军
HUANG Xiang-ling;LIN Fei-fei;ZHANG Ming-yue;ZHU Li-qiong;ZHAO Li-jun(College of Forestry,Guangxi University,Nanning 530004,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2018年第7期1364-1369,共6页
Journal of Southern Agriculture
基金
国家自然科学基金项目(31560061)
关键词
小叶榄仁
盐胁迫
生长指标
渗透性调节物质
Terminalia neotaliala Capuron
salt stress
growth index
osmoregulatory substances
作者简介
黄相玲(1992-),研究方向为森林生态与植物生理学,E-mail:1205453404@qq.com。;通讯作者,招礼军(1970-),博士,教授,主要从事森林生态与植物生理研究工作,E-mail:zhlj-70@163.com。
