摘要
目的:建立快速测定肉桂药材水分的方法。方法:采用甲苯法测定药材样品含水量(作为参考值)。采用近红外光谱法结合偏最小二乘法建立药材样品水分的定量模型。根据药材样品水分测定参考值,采集80批药材样品,以常数偏移消除法预处理光谱,药材样品水分测定最佳波段范围为7 502.2~6 896.6、5 600.6~4 998.9 cm^(-1)。结果:药材样品水分定量模型的内部交叉验证决定系数(R^2)为0.974 9,交叉验证均方差为0.404;模型外部验证R^2为0.940 4,预测均方差为0.306。结论:该方法快速准确,简便无污染,可用于肉桂药材水分的快速测定。
OBJECTIVE:To develop a method for rapid determination of water content in Cinnamomum cassia.METHODS:The moisture of sample was determined by toluene method(as reference value).IFRS-PLS was used to establish quantitative correction model of moisture in sample.According to moisture determination of sample,80 batches of samples were collected,and constant offset elimination method was used to pretreat spectrum.The optimal spectrum range for moisture determination of medicinal sample were 7 502.2-6 896.6 cm-1 and 5 600.6-4 998.9 cm-1.RESULTS:The internal cross validation decision coefficient(R2)of moisture quantitative correction model was 0.974 9,and the root mean square error of cross validation(RMSECV)was 0.404.R2 of model external validation was 0.940 4,and the predicted root mean square error was 0.306.CONCLUSIONS:The method is rapid,accurate,simple and free from pollution.It can be used for rapid determination of moisture in C.cassia.
作者
秦斌
闫研
殷果
庾红萍
王炳志
王铁杰
QIN Bin;YAN Yan;YIN Guo;YU Hongping;WANG Bingzhi;WANG Tiejie(Shenzhen Institute for Drug Control/Shenzhen Key Lab of Drug Quality Standard Research,Guangdong Shenzhen 518057,China;Shenzhen Bioeasy Technology,Inc.,Guangdong Shenzhen 518101,China)
出处
《中国药房》
CAS
北大核心
2018年第14期1949-1953,共5页
China Pharmacy
基金
国家药品抽验项目(No.中检监督函[2017]16号-101)
关键词
肉桂
近红外光谱法
水分
定量模型
Cinnamomum cassia
Near-infrared spectroscopy
Moisture
Quantitative model
作者简介
秦斌,副主任药师。研究方向:药物分析与质量标准。E-mail:dinal29@126.com。;殷果,通信作者:主任药师。研究方向:药品质量控制。E-mail:ayinguoa@126.com。
