摘要
【目的】为明确引起白及叶片产生黑褐色轮纹状干枯的病原菌。【方法】将采自云南省富宁县的该病害样品进行分离纯化,并通过初步形态学鉴定;进一步通过柯赫氏法则再次验证分离得到的菌株是否能引起白及发病;通过形态学观察和r DNA-ITS序列分析的方法鉴定该菌。【结果】该菌株能引起白及发病,并最终鉴定出该菌为黑轮层炭壳(Daldinia sp),菌株编号BJ-HLCTK。显微结构观察结果:中间黑褐色,边缘灰白色,同心轮纹,贴基生长,气生菌丝生长较慢,菌丝呈绒毛状;菌丝有隔膜,具分枝,宽9~20μm,平均宽13μm。孢子不等边椭圆形或肾脏形,11~16(17.8)μm×6~9(10.2)μm。ITS序列与黑轮层炭壳菌(Daldinia consentrica)同源性最高,相似度达97.1%。【结论】菌株BJ-HLCTK属于炭球菌属(Daldinia sp.),与该属真菌黑轮层炭壳种(D.consentrica)最为接近。该菌主要危害多种阔叶树种,被害木质部形成杂斑腐朽。本研究首次报道该病原菌自然侵染白及。
[Purpose]In order to clear the pathogen causing leaf lesion with brown-black circle spot of Bletilla striata.[Method]This study was isolated and purified the sample of B.striata collected from Funing County,Yunnan Province,through morphological identification and verification of Koch's Postulates to verify if the strain could cause the same diease of B.striata.[Result]The strain could cause the same diease of B.striata as the sample,and it was finally identified as Daldinia sp.by morphological observation and rDNA-ITS sequence analysis.The strain was numbered BJHTKLC,the colony morphology and microstructure were described as following:black brown in middle,marginal gray,concentric whorl,attachment base growth,aerial mycelium growth slower,hyphae villous;hyphae with septa,branched,9-20μm wide,average 13μm wide,spore morphology were elliptical or kidney line,11-16(17.8)μm×6-9(10.2)μm.ITS sequence had the highest similarity to D.concentrica,which was 97.1%.[Conclusion]BJ-HLCTK belongs to the genus of Daldinia sp.,and which has the highest close to fungus D.concentrica species(D.consentrica).The fungus is mainly harmful to a variety of broad-leaved tree species and damaged xylem to form mottled rot.In this study,we first reported that the host of this pathogen was Rhizoma Bletillae.
作者
柯尚艳
杨林毅
陈潞
孙媛
李孟霞
文国松
赵明富
徐绍忠
KE Shangyan;YANG Linyi;CHEN Lu;SUN Yuan;WEN Guosong;WEN Guosong;ZHAO Mingfu;XU Shaozhong(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Station of Plant Protection and Quaratine in Wenshan Prefecture,Wenshan 663099,China;Standardized Production Technical Guidance Center of Chinese Medicinal Materials,Yunnan Agricultural University,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第3期405-409,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省科技惠民项目(2015RA083)。
关键词
白及
黑轮层炭壳菌
分离与鉴定
Bletilla striata
Daldinia consentrica
isolation and identification
作者简介
第一作者:柯尚艳(1992—),女,陕西安康人,在读硕士研究生,主要从事植物病理研究。E-mail:2418842523@qq.com;;第一作者:杨林毅(1992—),男,河南林州人,在读硕士研究生,主要从事植物病理研究。E-mail:13503804902@163.com。;通信作者:赵明富(1975-),男,云南宣威人,博士,副教授,主要从事植物病理和微生物研究。E-mail:zhaomingfu@163.com;;通信作者:徐绍忠(1969—),男,云南昆明人,博士,副教授,主要从事中药材研究。E-mail:2737308675@qq.com。
