摘要
目的:分析miR-144靶基因在肺鳞癌中参与的信号通路生物过程。方法:分析The Cancer Genome Atlas网站中肺鳞癌患者的miR-144的表达量及生存曲线;预测miR-144靶基因并通过表达数据筛选影响肺鳞癌进展基因;BinGo软件对候选靶基因进行GO注释分析,利用DAVID网站预测其信号通路。结果:miR-144表达量高有利于延长肺鳞癌患者生存时间。综合4个靶基因数据库发现miR-144有72个预测结果,其中45个在肺鳞癌患者癌与癌旁组织间表达存在差异。其中部分候选靶基因参与胞核及星形微管的组成,参与上皮细胞增生、RNA合成。通路分析显示部分候选靶基因参与Ras、Wnt、Hippo等信号通路。结论:45个miR-144候选靶基因通过多条信号通路及生物过程调节细胞生命活动,影响肿瘤患者生存预期。
Objective:To analyze the target genes of miR-144 in lung squamous cell carcinoma and their biological processes and signaling pathways.Methods:The relationship between miRNA expression and survival plot of patients with lung squamous cell carcinoma in TCGA was analyzed.And then the target gene of miR-144 was predicted by target gene databases.The gene ontology(GO)enrichment was performed by BinGo.DAVID database was used to predict the target gene signal pathway.Results:The high expression of miR-144 was beneficial to prolong the overall survival of patients with lung squamous cell carcinoma.Seventy-two target genes of miR-144 were obtained by 4 miRNA database and 45 of 72 target genes were significantly different in cancer and normal tissue.GO enrichment analysis found that genes constituted the nucleus and astral microtubule mainly.And they took part in endothelial cell proliferation and RNA.Ras,Wnt and Hippo signal pathway were affected by the target genes.Conclusion:Forty-five miR-144 target genes may affect cell life activities and regulate tumor development by multiple signaling pathways.
作者
李伟
王兆松
董秋萍
徐玥
陈永孜
许世磊
LI Wei;WANG Zhao-song;DONG Qiu-ping;XU Yue;CHEN Yong-zi;XU Shi-lei(Cancer Institute and Hospital,Tianjin Medical University,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin’s Clinical Research Center for Cancer,Tianjin 300060,China)
出处
《天津医科大学学报》
2018年第4期287-290,共4页
Journal of Tianjin Medical University
作者简介
李伟(1989-),男,硕士在读,研究方向:生物化学与分子生物学;通信作者:许世磊,E-mail:slxu@sibs.cn。
