摘要
目的检测巴马小型猪胚胎-胎仔发育毒性试验的生殖功能指标和胎仔的各项发育指标,为巴马小型猪的发育毒性研究提供参考数据。方法 12只雌性巴马小型猪分别于妊娠第30、40、50、60、70、80、90、100、112天剖检胎仔,计数黄体数、着床数、活胎数、吸收胎数和死胎数,测量胎仔身长、尾长、脑直径,X光拍照后进行内脏和骨骼检查。结果巴马小型猪妊娠40 d胎仔性别可辨,内脏器官、骨骼系统基本发育成型。得到妊娠不同天数的小型猪胎仔各项发育指标的正常值和标准差,及95%的可信区间。结论巴马小型猪可以作为胚胎-胎仔发育毒性试验的观察对象,此次研究得到这些基础数据可以为评价外源化合物的生殖毒性试验提供背景参考数据。
Objective To obtain the basic data of Bama minipigs and provide basic reference for embryo-fetal developmental toxicity study.Methods Pregnant minipigs were sacrificed at different days during the gestation period respectively.The examinations included necropsy,count of corporea lutea,live and dead implantations,fetal body weight,and external,visceral,and skeletal examination of fetuses.Results The basic data of Bama minipigs,such as body weight,fetal development,and fetal malformation/variation were obtained.Conclusions We obtained the basic reproductive parameters of pregnant Bama minipigs and the indexes of fetal development,which can provide valuable reference data for embryo-fetal developmental toxicity tests of Bama minipigs.
作者
张元慧
郝新彦
高虹
ZHANG Yuanhui;HAO Xinyan;GAO Hong(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences;Comparative Medical Center,Peking Union Medical College,Beijing 100021,China)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第4期81-87,共7页
Chinese Journal of Comparative Medicine
关键词
巴马小型猪
胚胎发育
生殖毒性试验
Bama minipig
embryonic development
reproductive toxicity study
作者简介
张元慧(1993—),女,硕士研究生,专业:比较医学。E-mail:369098717@qq.com;[通信作者]高虹(1968—),女,研究员,研究方向:药物临床前安全性评价。E-mail:gaoh@cnilas.org
