摘要
以‘明淮2号’山药为试验材料,进行山药快繁技术条件的优化研究。结果表明:初代培养的外植体以中部带芽茎段为好。最好的消毒方法是用70%的酒精浸泡10s,0.1%HgCl_2浸泡10min,污染率为15.0%,死亡率为6.7%。初代培养基为:MS+6-BA1.0mg·L^(-1)+NAA0.05mg·L^(-1)+PVP100mg·L^(-1)+VC 500mg·L^(-1)+AC0.5g·L^(-1)+蔗糖30g·L^(-1),2周后转入相同配方的固体培养基中,褐化率为36.7%,诱导率为81.7%。增殖培养基为:MS+6-BA2.0mg·L^(-1)+KT1.0mg·L^(-1)+NAA0.05mg·L^(-1)+PP_(333)0.05mg·L^(-1)+琼脂粉4.0g·L^(-1)+蔗糖30g·L^(-1),30d后增殖系数为2.97。生根培养基为:1/2MS+NAA0.3mg·L^(-1)+IBA0.2mg·L^(-1)+AC1.0g·L^(-1)+琼脂粉4.5g·L^(-1)+蔗糖20g·L^(-1),30d生根率达91.3%。组培苗炼苗2周,取出用多菌灵浸泡后移栽到草炭土穴盘中,成活率达70%以上。
With‘Ming Huai yam 2’as the experimental material,we optimized the technological conditions of the rapid propagation of yam.The results showed that in vitro culture,the best explant was segment with bud in middle part of new shoot.The best disinfection method was with 70%alcohol immersion 10 s,and 0.1%HgCl 2 for 10 min,the pollution rate of which was 15.0%,and the mortality rate was 6.7%.The primary culture medium was:MS+6-BA 1.0 mg+NAA 0.05 mg·L-1+PVP 100 mg·L-1+VC 500 mg·L-1+AC 0.5 g·L-1+Sug 30 g·L-1.After two weeks,it was transferred to the solid medium with the same formula.The browning rate was 36.7%and the induction rate was 81.7%.The subculture multiplication medium was:MS+6-BA 2.0 mg·L-1+KT 1.0 mg·L-1+NAA 0.05 mg·L-1+PP 333 0.05 mg·L-1+Agar 4.0 g·L-1+Sug 30 g·L-1.The proliferation coefficient of 30 d was 2.97.The rooting medium was:1/2MS+NAA 0.3 mg·L-1+IBA 0.2 mg·L-1+AC 1.0 g·L-1+Agar 4.5 g·L-1+Sug 20 g·L-1.The rooting rate was 91.3%.After two weeks of acclimation in the greenhouse,the plants were soaked with carbendazim and cooled to dry,then transplanted to peat soil tray.The transplanting survival rate was more than 70%.
作者
陈芝华
华树妹
李丽红
罗晓锋
贺佩珍
CHEN Zhi-hua;HUA Shu-mei;LI Li-hong;LUO Xiao-feng;HE Pei-zhen(Sanming Academy of Agricultural Sciences,Sanming,Fujian 365051,China)
出处
《福建农业学报》
CAS
北大核心
2018年第2期164-170,共7页
Fujian Journal of Agricultural Sciences
基金
福建省农业引导性(重点)项目(2017N0051)
福建省星火科技计划项目(2016S0035)
关键词
山药
组织培养
快繁体系
优化
chinese yam
tissue culture
rapid propagation system
optimization
作者简介
陈芝华(1980-),男,硕士,助理研究员,主要从事作物育种研究工作(E-mail:czh_5555@163.com)。;通讯作者:华树妹(1978-),女,副研究员,主要从事山药育种及栽培技术研究(E-mail:hsm20082006@126.com)。
