摘要
文章以孵化14d的鸡胚为原料,采用缓冲液法对鸡胚中的蛋白质进行提取研究,评价了其体外抗氧化活性并分析了其氨基酸组成。采用乙醚低温回流脱去鸡胚干粉中脂肪,通过差示扫描量热仪(differential scanning calorimetry,DSC)确定粗蛋白的变性起始温度为62.47℃。在单因素的基础上,应用Box-Behnken中心组合实验设计建立数学模型,得到了最优提取条件为料液比1∶16、温度50℃、时间116min,此条件下鸡胚蛋白提取率为73.87%。按此方法提取的鸡胚蛋白对1,1二苯基-2-三硝基苯肼自由基(DPPH·)、羟基自由基(·OH)具有较强的清除能力。鸡胚蛋白中必需氨基酸组成齐全,含量高,是一种理想的动物蛋白资源,有待进一步开发。
Protein extraction from fertilized egg on 14 d with a buffer solution method was studied.Amino acid composition was analyzed and the in vitro antioxidant activity of fertilized egg protein(FEP)was also evaluated.The fat was removed by diethyl ether with a method of reflux at low temperature,the denaturation temperature of the FEP was 62.47℃with differential scanning calorimetry(DSC).Based on single factor experiment,the mathematical model was established by Box-Behnken center composite design.The results showed that the optimum conditions were as follows:the ratio of solid/liquid was 1∶16,the temperature was 50℃and the time was 116 min.Under these conditions,the extraction rate of protein from fertilized egg was 73.87%.The scavenging activity against l,l-diphenyl-2-picrylhydrazyl(DPPH·)and hydroxyl radical(·OH)of the FEP was strong.FEP was rich in essential amino acid,and the amino acid composition of it was comparatively complete,which indicated that FEP was a kind of ideal animal protein resource and should be further developed.
作者
唐正江
汤飞
吴娱
王莹
王碌碌
刘国庆
TANG Zhengjiang;TANG Fei;WU Yu;WANG Ying;WANG Lulu;LIU Guoqing(School of Food Science and Engineering,Hefei University of Technology,Hefei 230009,China)
出处
《合肥工业大学学报（自然科学版）》
CAS
北大核心
2018年第4期545-552,共8页
Journal of Hefei University of Technology：Natural Science
基金
安徽省皖江禽产业研究院公共服务平台资助项目(1401032006)
关键词
鸡胚
响应面
工艺优化
抗氧化
氨基酸分析
fertilized egg
response surface
process optimization
antioxidant
amino acid analysis
作者简介
唐正江(1991-),男,安徽宿松人,合肥工业大学硕士生;通讯作者:刘国庆(1963-),男,安徽合肥人,博士,合肥工业大学教授,硕士生导师,E-mail:Liugq168@163.com.
